突触后致密蛋白95(PSD95)和突触小泡蛋白在神经元成熟过程中的分布

Distribution of postsynaptic density protein 95(PSD95) and synaptophysin during neuronal maturation

目的探讨突触后致密蛋白95(PSD95)和突触小泡蛋白(SYP)在原代培养不同时间神经元中的表达。方法采用免疫荧光技术检测原代培养3 d(3DIV)、7DIV和14DIV大脑皮层神经元内的PSD95和SYP的表达。结果 PSD95在3DIV时主要分布在神经元胞体;7DIV时分布在胞体、突起末端和分支处;14DIV时分布在胞体和呈斑点状分布在神经元突起上。SYP在3DIV时无明显表达,7DIV时分布在神经元细胞核内,14DIV时分布在细胞核内和呈斑点状分布在突起上。结论随着培养神经元和突触的发育至成熟,PSD95和SYP最初主要位于神经元胞体和细胞核内,最终大都呈斑点状密集分布在突起上。表明PSD95和SYP虽产生部位不同,但最终都与突触的形成和成熟密切相关。

Objective To investigate the expressions of postsynaptic density protein 95 （PSD95） and synaptophysin （SYP） in primary culture of neurons at different times. Methods The expressions of PSD95 and SYP were detected using immunofluorascence technique in primary culture of cerebral cortical neurons at 3 days in vitro （3DIV）, 7DIV and 14DIV. Results PSD95 was mainly distributed in the cell body at 3DIV, in the cell body, protuberance end and branch of neurons at ？DIV, and in the cell body as well as protuberance with a stippled pattern at 14DIV. The expression of SYP was not obvious at 3DIV, and it was seen mainly in nucleus at 7DIV, and in nucleus and protuberance showing a stippled pattern at 14DIV. Conclusion PSD95 and SYP are mainly located in the soma and nucleus of neurons in the early stage of neuron maturation, and finally mostly move to the protuberance with a stippled pattern, which indicates that PSD95 and SYP may originate from the different sites, but they are closely related to the formation and maturation of synapse.