(Pyr^1)apelin-13对Jarkat T淋巴细胞增殖和活化的影响

Effects of(Pyr^1) apelin-13 on the proliferation and activation of Jarkat T lymphocyte

目的探讨(Pyr^1)apelin-13对Jurkat T淋巴细胞增殖和活化的影响。方法用(0、10、50)nmol/L(Pyr^1)apelin-13处理Jurkat T淋巴细胞24 h后,用CCK-8法检测细胞增殖能力的变化;用抗CD3抗体和抗CD28抗体包被并活化Jurkat T淋巴细胞,观察(Pyr^1)apelin-13对Jurkat T淋巴细胞活化的影响;实时定量PCR检测CD69、CD25、细胞毒性T淋巴细胞相关蛋白4(CTLA-4)及程序性死亡蛋白1(PD-1)的mRNA水平。结果 (Pyr^1)apelin-13对Jurkat T淋巴细胞增殖无影响,但通过增加CD69和CD25的表达促进Jurkat T淋巴细胞活化,但对CTLA-4和PD-1表达影响不明显。结论 (Pyr^1)apelin-13促进Jurkat T淋巴细胞活化。

Objective To investigate the effect of （Pyr1 ） apelin-13 on the proliferation and activation of Jurkat T lymphocytes. Methods Jurkat T cells were treated by 0, 10, 50 nmol/L （Pyr1） apelin-13 for 24 hours and cell proliferation ability was detected by CCK-8 assay. The effect of （ PyrI ） apelin-13 on the activation of Jurkat T lymphocytes with anti-CD3 and anti-CD28 antibodies was observed and the molecular mechanism was investigated. Quantitative real-time PCR was applied to detect the expressions of CD69, CD25, cytotoxic T lymphocyte antigen-4 （CTLA-4） and programmed death-t （ PD-1 ） mRNAs. Results The proliferation of Jurkat T cells was not affected by （Pyr1） apelin-13. However, （Pyr1） apelin-13 increased the expressions of CD69 and CD25 and then activated Jurkat T lymphocytes. Furthermore, the expressions of CTLA-4 and PD-] had no difference between activation group and （ Pyrt ） apelin-13 group. Conclusion （ Pyr1 ） apelin-13 promotes Jurkat T lymphocyte activation by increasing the expressions of CD69 and CD25.