电针长强穴对血管性痴呆大鼠学习记忆能力及海马CA1区细胞凋亡的影响

Effect of Electroacupuncture at Changqiang Acupoint on Learning and Memory Ability and Hippocampal CA1 Apoptosis in Rats with Vascular Dementia

目的:观察电针长强穴对血管性痴呆(VD)大鼠学习记忆能力及海马CA1区细胞凋亡的影响。方法:将水迷宫筛得的60只雄性大鼠按照随机数字表分为空白组9只、假手术组9只和手术组42只。手术组采用双侧颈总动脉永久性结扎法制备模型,术后1周筛得27只,随机分为模型组9只、电针长强穴组9只、电针非穴组9只,干预后行水迷宫测试及原位末端转移酶标记技术检测。结果:空白组和电针长强穴组的逃避潜伏期、CA1区细胞凋亡数低于模型组(P<0.05),穿越平台次数多于模型组(P<0.05);电针长强穴组CA1区细胞凋亡数与逃避潜伏期正相关,与穿越平台次数负相关(P<0.05)。结论:电针长强穴能够提高VD大鼠学习记忆能力及抑制海马CA1区细胞凋亡,且两者相关。

Objective：To observe the effect of electro-acupuncture（EA） at Changqiang（GV1） acupoint on the learning and memory ability and hippocampal CA1 cellular apoptosis in rats with vascular dementia（VD）. Methods：Sixty male SD rats according to the Morris water maze test were divided into three groups：9 rats for blank control group,9 rats for sham-operated group and 42 rats for surgical control group. Rats of VD models were established by 2 vessels obstruction（ 2-VO）, 27 rats were selected from the surgical control group and divided into three groups：9 rats for model group,9 rats for EA treatment group and rest 9 rats for the non-acupoint EA control group. After the treatment,the water maze test and the TUNEL test were examined. Results：Compared with the model group,the escape latent period and the CA1 apoptosis of the blank control group and the EA treatment group were apparently shorter（P〈0.05）,and the times of crossing the platform were significantly increased（P〈0.05）. A positive correlation between CA1 apoptosis and latent period was identified（P〈0.05）,a negative correlation between apoptosis and the times of crossing was identified（P〈0.05）. Conclusion：EA at Changqiang acupoint may improve the learning and memorizing ability and restrain the apoptosis of hippocampal CA1 region of VD rats,and association of the ability and apoptosis is positive.