电针曲池、足三里穴对大脑中动脉阻塞大鼠缺血周围纹状体区神经细胞自噬的影响

Effect of Electroacupuncture at Quchi and Zusanli Acupoints on Peri-infract Striatal Neural Autophagy in MCAO Rats

目的:观察电针曲池、足三里穴对大脑中动脉阻塞(MCAO)缺血再灌注损伤大鼠缺血周围纹状体区神经细胞自噬的影响。方法:将36只SD大鼠按照随机数字表分为假手术组、模型组和电针组各12只。参考Koizumi线栓法构建MCAO大鼠模型后,电针组采用电针曲池、足三里穴干预3 d,假手术组和模型组不予以电针刺激。TTC染色观察梗死体积,电镜观察自噬体和线粒体结构,并检测自噬标志蛋白微管相关蛋白1轻链3B(1ight chain 3B,LC3B)的表达水平。结果:TTC染色显示,电针组MCAO大鼠脑梗死体积较模型组减少(P<0.05);电镜观察发现,模型组溶酶体和自噬泡数量增加,而电针组该现象减弱;电针组LC3BⅡ/LC3BⅠ水平较模型组升高(P<0.05)。结论:在脑缺血再灌注损伤亚急性期,电针曲池、足三里穴可抑制缺血周围纹状体区因溶酶体和自噬溶酶体过度释放引起的神经细胞自噬性死亡,其机制还有待进一步研究。

Objective：To observe the effects of electroacupuncture（EA） at the Quchi（LI11） and Zusanli（ST36） acupoints on peri-infract striatal neural autophagy in middle cerebral artery occlusion（MCAO）-induced ischemia and reperfusion injured rats.Methods：Thirty-six SD rats were divided into the sham group,the MCAO group and the EA group by a random number table,12 rats in each group. After the MCAO models were established according to the Koizumi method,the rats in the EA group received EA at the LI11 and ST36 acupoints intervention with 3 days. The infract volume of cerebral ischemia were measured by TTC staining. The number of autophagosome and the structure of mitochondria were evaluated by electron microscope（EM）.The expression of autophagy marker microtubule-associated protein 1ight chain 3B（LC3B） were detected. Results：TTC staining showed that the infarct volumes of rats in the EA group were reduced compared to the sham group（P〈0.05）. The EM showed that the number of lysosomes and autolysosome were increased in the MCAO group,and after EA intervention,the phenomenon that increased lysosomes and autolysosome was weakened compared to the MCAO group. The levels of LC3 B Ⅱ/ LC3 B Ⅰ in the EA group were increased compared to the MCAO group（P〈0.05）. Conclusion：In the subacute stage of cerebral ischemic injury,EA at the LI11 and ST36 acupoints could alleviate autophagic cell death induced by excessive release of lysosomes and autolysosome in peri-infract striatum,the mechanisms of which need to be studied further.