姜黄素对沙漠干热环境中暑大鼠脑损伤的保护作用研究

Study of Curcumin Neuroprotective Effects on Heatstroke Rats in Dry-heat Desert Environment

目的观察姜黄素对沙漠干热环境中暑大鼠脑组织氧化应激的影响。方法 200只SD大鼠,随机分为5组:盐水对照组、溶剂组、姜黄素预处理低剂量组、姜黄素预处理中剂量组、姜黄素预处理高剂量组。各组连续7 d等体积灌胃,同时置于西北特殊环境人工实验舱,设定沙漠干热环境气候模式温度(41±1)℃,湿度10%RH。分别在0 min、50 min、100 min、150 min各组随机取10只动物检测脑组织丙二醛(MDA)及谷胱甘肽(GSH)的含量。结果(1)0 min时间点各组大鼠脑组织MDA的含量无显著性差异(P>0.05),随着干热环境持续时间延长MDA含量呈现上升趋势,姜黄素预处理高剂量组与对照组、溶剂组差异具有显著性(P<0.05)。(2)0 min时间点各组大鼠脑组织GSH的含量无显著性差异(P>0.05),随着干热环境持续时间延长GSH含量逐渐下降,姜黄素预处理高剂量组与对照组、溶剂组差异具有显著性(P<0.05)。结论姜黄素对沙漠干热环境中暑大鼠脑组织氧化应激损伤具有抑制作用,对神经系统具有一定保护作用。

Objective To study the neuroprotective effect of Curcumin on the oxidative stress indexes of heatstroke rats in dry-heat environment of desert. Method The 200 male SPF SD rats, were randomly divided into control group, low-dose Cureumin pretreated group, middle-dose Curcumin pretreated group, high-dose Curcumin pretreated group and solvent group, each group had 40 rats. All the rats were put into the dry heat environment （temperature of （41±1）℃ , humidity 10% ） in The Simulated Climate Cabin for Special Environment of Northwest of China. Each group were randomly selected 10 rats to measure oxidative stress indexes-brain malondialdehyda （MDA） and glutathione（GSH） levels at the time of 0 min, 50 min, 100 min, and 150 min. Result There were no significant difference of MDA and GSH levels among the groups at 0 min（P 〉0.05）. The MDA levels of rat＇s brain tissue increased and GSH levels reduced with prolonged in dry heat environment. The MDA and GSH levels of brain tissue of high dose Curcumin pretreatment group compared with the control group and the solvent group, were significantly different （P 〈 0.05 ）. Conclusion Cureumin can reduce the MDA levels of brain tissue and increase the GSH level of heatstroke rats in dry heat environment of desert. Curcumin has a protective effect on the heatstroke rats in the dry heat environment of desert.