维生素B_2联合紫外光照射光化学法对血小板源性细胞因子释放的影响

Effects of VB_2-photochemical technology on the release of platelet-derived cytokines during apheresis platelets storage

目的探讨病原体灭活技术(PRT)是否对血小板源性细胞因子的释放产生影响。方法随机选取捐献血小板的献血者20人,取单采血小板标本60 m L/人(份),全部平均分成2份(组),实验组:20份采用维生素B2(终浓度为50μmol/L)联合紫外光照射(波长265-370 nm,剂量6.2 J/m L)光化学法照射8.5 min;对照组:20份不做处理。2组标本均放置于(22±2)℃水平振荡条件下保存7 d,分别于1、3、5和7 d取样6 m L,检测其血小板计数(Plt)、血小板分布宽度(PDW)、平均血小板体积(MPV)以及血小板源性细胞因子(CCL3、CCL5、TGF-β-1和PF4)含量。结果在保存的7 d过程中,与对照组相比,实验组中Plt略微降低,MPV和PDW略微升高(P>0.05)。实验组与对照组比较,在7 d时,CCL3含量(pg/m L)为11.90±0.4 vs 10.97±0.51(P<0.05),CCL5含量(ng/m L)为190.10±15.03 vs 150.02±20.0(P<0.05);在5和7 d时,TGF-β-1含量(ng/m L)分别为22.18±2.36 vs 21.15±1.43、37.21±3.01 vs 34.11±2.03(P<0.05),PF4含量(mg/L)分别为7.87±1.05 vs 5.75±0.91、9.01±1.11 vs 6.13±1.07(P<0.05)。结论随着血小板贮存时间的延长,血小板源性细胞因子的积累逐渐增加;在血小板保存末期,VB2-PRT处理明显增加血小板源性细胞因子的积累。

Objective To investigate whether pathogen reduction technology （PRT） treatment may lead to increase platelet-derived cytokines release from platelets during storage. Methods Sixty milliliters of leukoreduced apheresis platelets were collected from 20 healthy donors, respectively. Then splitting was performed： one part （30mL/each） was treated with vitamin B2（ work concentration 50 Ixmol/L）-UVB （6. 2 J/mL） photochemical technology as experimental group （20 individ- uals） , while the other remained untreated to serve as control （20 individuals）. During 7 d of storage, the platelets were an- alyzed for platelet counting （Ph）, platelet distribution width （PDW）, mean platelet volume （MPV） and platelet-derived cytokines （ CCL3, CCL5, TGF-β-1 and PF4） on day 1, 3, 5 and 7 of storage. Results No significant differences were ob- served on Pit, PDW and MPV between the experimental and control groups during 7 d storage, respectively. The CCL3 con- tent [ （11.90±0.4） pg/mL] in the experimental group was significant higher than that [ （ 10. 97±0. 51）pg/mL] in the con- trol（P〈0. 05）on the 7 d of storage. The CCL5 content [ （ 190. 10±15.03） ng/mL] in the experimental group was significant higher than that [ （ 150.02±20. 0）ng/mL] in the control（P〈0. 05）on the 7 d of storage. The TGF-β-1 content（ng/mL） in experimental group was 22. 18±2. 36 and 37. 21±3.01, significant higher than those （5.75±0. 91 and 6. 13±1.07） in the control（P〈0. 05）on the 5 and 7 d of storage, respectively. The PF4 content（mg/mL） in experimental group was 7. 87±1.05 and 9. 01 ± 1.11, significant higher than those （ 5.75 ± 0. 91 and 6. 13 ± 1.07 ） in the control （ P〈 0. 05 ） on the 5 and 7 d of storage, respectively. Conclusion The levels of platelet-derived cytokines increase in both groups during platelets storages. The PRT treatment leads to significantly increase the accumulation of platelet-derived cytokines at end of storage.