化橘红对大鼠糖尿病心肌病的防治作用及其机制研究

Prevention and cure effect of xocarpium Citri Grandis in diabetic cardiomyopathy rats and its mechanisms

目的探讨化橘红提取物(extract of exocarpium citri grandis)对链脲佐菌素(STZ)诱导大鼠糖尿病心肌病的防治作用及其机制。方法 SD大鼠建立实验性2型糖尿病心肌病模型,随机分为模型对照组、化橘红高、中、低剂量治疗组、PPARγ激动剂吡格列酮治疗组(每组各15只),正常大鼠15只作为正常对照。观察不同剂量化橘红口服10、20、40mL/(kg·d)治疗对心肌结构功能损伤程度的影响;HE染色检测心肌组织结构改变,免疫印迹法检测心肌组织p 38 MAPK和cleaved caspase-3的表达,透射电镜观察心肌细胞超微结构。结果与正常对照组比较,模型组大鼠心脏组织心肌凋亡增多,HE染色可见心肌肥大、胶原沉积,心肌细胞超微结构损伤显著;化橘红干预组能减轻心肌凋亡、心肌肥大、胶原沉积等,同时能明显抑制p-p38 MAPK和cleaved caspase-3的表达。结论化橘红能防治糖尿病心肌病心肌结构功能损伤,其机制可能与抑制心肌p38 MAPK信号通路有关。

Objective To investigate prevention and cure effect of of exocarpium citri grandis（ECG） in streptozocin-induced diabetic cardiomyopathy（DCM） rats and its mechanisms. Methods Sprague-Dawley（SD） Rats were divided into DCM group,control group,ECG group（low, medium, high dose）,pioglitazone group.After intragastric administration with different concentrates of ECG,myocardial histopathologic changes were measured using haematoxylin and eosin staining,p 38 MAPK and cleaved caspase-3 levels were tested through Western blot assay.Ultrastructure of myocardial tissues were detected by means of electron microscopy. Results Compared with control group,myocardial cells apoptosis,hypertrophy,collagen deposit and ultrastructural changes in heart tissues were exacerbated,administration with ECG improved these injured alternations and inhibited expressions of p-p38 MAPK and cleaved caspase-3. Conclusion ECG can prevent and treat diabetic cardiomyopathy myocardial structural damage.Its mechanisms might mediate by p 38 MAPK signal pathway.