铬胁迫对葎草雌雄叶片抗氧化酶活性及丙二醛含量的影响

Effect of Cr( Ⅲ) Stress on Antioxidative Enzymes Different Influence of Male and Female Humulus scandens Leaves

采用盆栽法研究不同铬浓度(Cr^(3+))(分别为0、50、200、300、500 mg/kg)下葎草(Humulus scandens)雌雄叶片抗氧化酶活性的不同影响。结果表明:1)Cr^(3+)胁迫显著影响了葎草雌雄幼苗的SOD、POD、CAT、Pr和MDA含量(P=0.002,P=0.001,P=0.002,P=0.000,P=0.000),总体上呈现出低促高抑的变化趋势。2)对照处理下,葎草雌株的超氧化物歧化酶(SOD)活性较雄株高,而丙二醛(MDA)含量、过氧化氢酶(CAT)、过氧化物酶(POD)活性和可溶性蛋白(Pr)含量则比雄株低。3)葎草雌雄幼苗的抗氧化酶活性在低浓度Cr^(3+)胁迫得到了促进,在中度Cr^(3+)胁迫(200~300 mg/kg)下受到显著抑制,膜质过氧化作用的显著增强,且高浓度Cr^(3+)胁迫(500 mg/kg)条件下,葎草幼苗雌雄株的抗氧化活性显著失衡,其抗氧化酶指标的变化出现差异。随着Cr^(3+)胁迫的增加,葎草雌雄幼苗SOD、POD和Pr含量呈先高后低的变化趋势,CAT呈下降趋势,而MDA却呈上升趋势,葎草雌株比雄株具有更积极的响应策略和更强的耐Cr^(3+)机制。

This paper studied the sex-specific differences of male and female Humulus scandens leaves in their antioxidative enzymes under the stress of chromium（ 0,50,200,and 300 mg/kg Cr^3＋）. The results showed： 1） Cr^3＋stress significantly affected the male and female H. scandens seedling in SOD,POD,CAT,Pr and MDA content（ P =0. 002,P = 0. 001,P = 0. 002,P = 0. 000,P = 0. 000）,with the variation tendency of ＂low-promotion,high-inhibition＂; 2） In the control treatment（ CK）,companied with the males,the superoxide dismutase（ SOD） activity of the females was higher,but the malondialdehyed（ MDA） content,catalase（ CAT）,peroxidase（ POD） and the soluble protein（ Pr） content of the females was lower; 3） the antioxidant enzyme activity of male and female H. scandens seedling was promoted in low concentration of Cr^3＋stress（ 200 - 300 mg/kg）,and inhibited in moderate Cr^3＋stress（ 200 - 300 mg/kg）,as well as a significant imbalance in high concentration of Cr^3＋stress（ 500 mg/kg）,and the changes of their antioxidant enzymes were different. With increase of Cr^3＋stress,the activities of SOD,POD and Pr content of male and female H. scandens seedling showed the ＂first high and then low ＂variation tendency,CAT downward trend,but MDA upward trend. The females of H. scandens seedling has a more positive response strategy than males,and stronger resistance mechanism to Cr^3＋stress.