抗菌消炎妇科中药的筛选及临床前实验研究

Screening of Chinese herbal medicine for antibacterial anti-inflammatory in department of gynaecology and preclinical experimental study

目的探讨妇科生殖系统感染的治疗药物,筛选抗菌消炎效果特别显著的中药。方法选择抗菌消炎的4种中药:树参、藤石松、松萝和石仙桃,分别用水提法和醇提法提取其组分,将其提取物配为相同浓度的溶液,分组为中药处理组(n=25)和水CK1、乙醇CK2对照组(n=25)。培养大肠埃希菌、金黄色葡萄球菌和产气大肠埃希菌,采用管碟法测定中药提取液的抑菌圈,评价抑菌效果。结果 4种中药水提液中,抑菌效果最好的是藤石松,对3种细菌的抑制效果都很明显,与对照组差异有统计学意义(P<0.01),树参对金黄色葡萄球菌的抑制效果较强,抑菌效果与对照组差异有统计学意义(P<0.05),其次是松萝,与对照组差异有统计学意义(P<0.05)。4种中药醇提液中,抑菌效果最好的是树参,对3种菌的抑制效果都很明显,与对照组差异有统计学意义(P<0.05),树参和松萝的醇提液抑菌效果几乎相当,与对照组差异有统计学意义(P<0.05)。结论树参和松萝醇提液、藤石松水提液的抑菌效果显著,适合在妇产科临床上外用,树参的水煎煮汁液口服,临床上均有抗菌消炎效果。

Objective To study the drug of treating gynecological reproductive system infection,and screen the Chinese herbal medicine with very good antibacterial anti-inflammatory effect.Methods Four kinds of Chinese herbal medicines were selected as the antibacterial and antiphlogistic drugs：Dendropanax dentiger Merr.,Lycopodium japonicum Thunb, Usnea diffracta Vain,Pholido-ta cantonensis Rolfe.They were extracted by water extraction and ethanol extraction,and then they were mixed into the same concentration solution.The group was the treatment group of Chinese herbal medicine （n=25）,and water CK1 and ethanol CK2 control group （n=25） respectively.Escherichia coil, Staphylococcus au- reus,Staphylococcus aureus were Cultured,and the bacteriostatic ring was determined of Chinese herbal medicine extract by tube plate method to evaluate the bacteriostatic effect.Results Among 4 kinds of water extract of Chinese herbal medicine,Lycopodium japonicum Thunb had the best bacteriostatic effect,and it had a very obvious bacteriostatic effect for 3 kinds of bacteria, and there was a statistical difference compared with control group （P〈0.O1）,and bacteriostatic ef- fect of Dendropanax dentiger Merr.was stronger for Staphylococcus aureus,and there was a statistical difference of bac- teriostatic effect compared with control group （P〈0.05）,Usnea diffracta Vain was following,and there was a statistical difference compared with control group （P〈0.05）.Among 4 kinds of alcohol extract of Chinese herbal medicine,Den- dropanax dentiger Merr.had the best bacteriostatic effect,and it had a very obvious bacteriostatic effect for 3 kinds of bacteria,and there was a statistical difference compared with control group （P〈0.05）,and bacteriostatic effect of alcohol extract for Dendropanax dentlger Merr. and Usnea diffracta Vain was almost quite,and there was a tatistical difference compared with comtrol group （P〈0.05）.Conclusion Bacteriostatic effect of alcohol extract of Dendrapanax dentiger Merr. and Usnea diffracta Vain and the water extract of Lycopodium japonicum Thunb is obvious which is suitable for external use in clinical obstetrics and gynecology.Oral water decoction juice of Dendropanax dentiger Merr. for oral admina has an antibacterial anti-inflammatory in clinic.