糖多酚A对辐射损伤小鼠的防护作用

Protective effect of sugar polyphenols A on radiation injury in mice

目的 通过建立137Cs γ射线诱导的辐射损伤小鼠模型，探讨通过结构修饰而得到的糖多酚A的辐射防护作用。 方法 4-6周龄的雄性昆明种小鼠42只，用随机数字表法分为阴性对照组（NCG）、阳性对照组（ＰCG）、照射对照组（ＩCG）以及糖多酚A的0.05、0.10和0.20 g/kg 3个剂量组。采用6.0 Gy 137Cs γ射线单次全身照射构建小鼠电离辐射损伤模型。射线照射前连续给药14 d，照射后继续给药7 d。照射后第7天处死小鼠，观察不同剂量的糖多酚A对小鼠脏器系数、外周血指标、脾结节数、骨髓DNA含量和股骨有核细胞数的影响。 结果 与ICG比较，糖多酚A 0.05、0.10、0.20 g/kg剂量组均可有效抑制照射后7 d小鼠免疫系统和造血系统的辐射损伤，使脾脏系数（Z＝-2.364、-3.003、-2.747，均Ｐ〈0.05）和内源性脾结节数均显著升高（Z＝ -2.458、-2.141、-2.206，均Ｐ〈0.05），其中糖多酚A 0.05 g/kg和0.20 g/kg剂量组白细胞数显著升高（Z＝-2.652、-2.139，均Ｐ〈0.05），糖多酚A 0.20 g/kg剂量组红细胞数（Z=-2.364，P〈0.05）、骨髓DNA含量（Z＝-2.492，Ｐ〈0.05）和股骨有核细胞数（Z＝ -2.492，Ｐ〈0.05）也显著高于ICG。 结论 糖多酚A可通过改善脏器系数，增高内源性脾结节数、外周血白细胞数、骨髓DNA含量和股骨有核细胞数来缓解电离辐射所致损伤，促进小鼠免疫和骨髓造血功能的恢复。

Objective To study the radiation protection effect of sugar polyphenols A obtained by structured modification using mouse model of radiation injury induced by ^137Cs γ-ray. Methods Total 42 male Kunming mice aged 4-6 weeks were randomly divided into six groups： negative control group （NCG）, positive control group （PCG）, irradiation control group （ICG） as well as three irradiation experimental groups. The radiation injury model was developed by once whole-body irradiation with 6.0 Gy ^137Cs γ-ray. The mice in three experimental groups were fed sugar polyphenols A respectively with doses of 0.05, 0.10 and 0.20 g/kg, for 14 d before irradiation, and continuously fed with the drug for 7 d after the irradiation. After that the mice were sacrificed, and the organ index, hematological parameters, spleen colony number, bone marrow DNA content and nucleated cells in bone-marrow were tested to evaluate the effect of sugar polyphenols A. Results Compared with the ICG, the irradiation experimental groups with the sugar polyphenols A doses of 0.05, 0.10, 0.20 g/kg showed reductions in irradiation injuries of immune system and hematopoietic system, which makes the significant increases of the spleen indexes （Z=-2.364, -3.003 and -2.747, P〈0.05） and the number of endogenous spleen colony （Z=-2.458, -2.141 and -2.206, P〈0.05）. In the groups with the doses of 0.05 and 0.2 g/kg, the number of white blood cells were significantly increased （Z=-2.652 and -2.139, P〈0.05）. Besides, in the group with the dose of 0.2 g/kg, the level of red blood cells （Z=-2.364, P〈0.05）, bone marrow DNA content （Z=-2.492, P〈0.05） and nucleated cells in bone-marrow （Z=-2.492, P〈0.05） were also increased compared with the ICG. Conclusions Sugar polyphenols A has the positive effects on the recovery of immune and hematopoietic function of mice through improving the organ index, endogenous spleen colony number, peripheral white blood cell number, bone marrow DNA content and nucleated cells in bone-marrow, which can reduce the injury induced by ionizing radiation.