CD98对肝癌细胞侵袭和转移的影响

Role of CD98 in invasion and metastasis of hepatocellular carcinoma cells

目的本研究拟通过RNA干扰技术下调人类肝癌细胞(hepatocellular carcinoma,HCC)CD98基因的表达,探讨CD98对肿瘤侵袭与转移的影响.方法利用CD98 siRNAs瞬时转染人类HCC系(FHCC-98),免疫印迹法证实癌细胞中CD98蛋白表达下调后,通过细胞黏附实验、Transwell侵袭实验及划痕愈合实验,检测CD98对HCC的黏附、侵袭和迁移能力的影响.结果 CD98 siRNAs瞬时转染FHCC-98细胞48 h后,癌细胞CD98的蛋白水平显著下调,干扰CD98蛋白表达水平后,癌细胞黏附能力显著下降,Transwell实验证实与对照组相比,癌细胞的侵袭能力显著下降,划痕实验也证实干涉CD98后癌细胞的迁移能力显著下降.结论 CD98通过增强HCC的黏附及迁徙能力,促进HCC的侵袭和转移,为未来肝细胞癌治疗提供了可能的靶点.

AIM To knock down CD98 expression in human hepatocellular carcinoma（HCC） cells by RNA interference（RNAi） to explore the role of CD98 in HCC progression.METHODS Down-regulation of CD98 expression in human HCC cell line（FHCC-98） was performed by RNAi and verified by Western blot analysis.The role of CD98 in the invasion and metastasis of FHCC-98 cells was investigated using cell adhesion assay,transwell invasion assay,and monolayer wound healing assay.RESULTS After transient transfection with siRNA targeting CD98 for 48 h,the protein expression of CD98 in human HCC cells was significantly knocked down.Moreover,the numbers of attached cells and invaded cells,and wound closure rate were significantly decreased compared to cells transfected with the negative control siRNA.CONCLUSION CD98 may promote tumor invasion and metastasis in HCC via enhancement of the abilities of tumor cells to adhere,invade and migrate.CD98 may be a novel therapeutic target for the treatment of HCC.