榄香烯对肝细胞癌的治疗作用及机制研究

Experimental Study for Elemene on Anticancer Effect and Mechanism of Hepatocellular Carcinoma

目的探讨榄香烯对肝细胞癌的抗癌作用及其机制。方法体外观察榄香烯处理后的小鼠肝癌细胞系H22细胞的凋亡及增殖影响,检测AKT相关基因的表达。建立小鼠肝癌原位移植模型,随机分A、B、C组,每组10只,A组生理盐水0.3ml/次/天,B组榄香烯3mg/0.3ml/次/日,C组替吉奥生理盐水0.2mg/0.3ml/次/日,连续灌胃两周,观察小鼠生活状态、体重变化及小鼠肝脏肿瘤大小。结果随着榄香烯浓度增大,H22细胞受抑制增殖的作用增强,半数抑制浓度约为30ug/ml,榄香烯处理后肝癌细胞AKT mRNA表达量没有明显的变化,AKT蛋白无明显变化,但是p-AKT蛋白明显降低。B组小鼠生活状态以及体重与A组无差别,肿瘤直径小于A组,B组小鼠生活状态及体重明显优于C组,肿瘤大小与C组无明显差别。结论体内外实验均提示榄香烯有抑制肝癌细胞生长的作用,其毒副作用不明显,其机制可能是通过PI3K/AKT途径,降低AKT的磷酸化抑制H22肝癌细胞增殖。

Objective To explore elemene＇ s anticancer effect and mechanism for the hepatocellular carcinoma. Methods treated by elemene, observing the apoptosis and proliferation of H22 cell, detecting the AKT related gene expression. Building the ortho- topic transplantation of liver cancer in mice model, separated randomly three groups,each group is ten mices. A group： saline group, 0. 3ml/time/day; B group： elemene group, 3mg/0. 3ml/time/day; C group： Tegafur Gimeracil Oteracil Potassium group, 0. 2mg/0. 3ml/time/day, in intragastric garage for two weeks, observing the mices＇ life state, weight and the liver tumor size. Results with the elemene concentration increasing, the stronger inhabiting the H22 cells proliferation, half inhibitory concentration was about 30ug/ml. The AKT mRNA and AKT protein expression had no obvious chan- ges for the H22 ceils treated by elemene, but the p-AKT protein was decreased obviously. There was no difference in living state and weight between group A and group B, while the group A and group B were superior to the group C. The tumor size in group B and group C were less than group A, that had the statistical significant. Conclusion Elemene inhabited the growth of liver cancer cells in vivo and vitro experiments, it had no obvious side effects, the mechanism may be through PI3K/AKT pathway, reduce the expression of p-AKT to inhabit the H22 cells proliferation.