4种天然药物对人肝癌细胞HepG2增殖抑制作用的比较

Comparison of Inhibitory Effect of Four Natural Medicines on Proliferation of HepG2 Cell Lines

目的比较吉马酮、芝麻素、淫羊藿素、褐藻多糖硫酸酯等4种天然药物对人肝癌细胞HepG2增殖的抑制效果,并探讨其作用机制。方法通过MTT法比较4种药物对HepG2细胞增殖的影响,流式细胞仪分析其对细胞凋亡的影响。Western blot法检测药物处理前后细胞增殖和凋亡相关蛋白的表达变化。结果 4种药物均呈浓度依赖性地抑制HepG2细胞的增殖,其中淫羊藿素对HepG2的抑制效果最为明显,作用48h半抑制浓度IC50为30μmol/L;4种药物均诱导HepG2细胞凋亡,淫羊藿素诱导细胞凋亡的效果最显著,30μmol/L淫羊藿素处理48h后,细胞凋亡率达到51.1%;4种药物均不同程度抑制周期相关蛋白Cyclin A、Cyclin B1、CDK1、CDK2和抗凋亡蛋白Bcl-2的表达,提高促凋亡蛋白Bax的表达。结论 4种天然药物在体外能明显抑制人肝癌细胞HepG2的增殖,诱导其凋亡,淫羊藿素效果最为显著;其分子机制与调节抑制细胞增殖和促凋亡相关蛋白的表达有关。

Objective To compare the effect of germacrone,sesamin,icaritin and fucoidan on proliferation of human hepatocellular carcinoma cell line HepG2 and study the underlying mechanism during those processes.Methods MTT assay was used to test the effect of the four natural medicines on proliferation of HepG2 cells.Apoptosis of the cells was detected by flow cytometry.The expression of several key proliferation-and apoptosis-related proteins was investigated by Western blotting.Results The four medicines inhibited the proliferation of HepG2 cells in a dose-dependent manner.The highest inhibition effect was induced by icaritin,and its IC50 of 48 htreatment was 30μmol/L.All the medicines induced apoptosis of HepG2 cells.Icaritin showed the most remarkable effect in inducing cell apoptosis.After treatment with 30μmol/L icaritin for 48 h,apoptosis rate reached 51.1%.The medicines reduced the expression of Cyclin A,CyclinB1,CDK1,CDK2 and Bcl-2,and increased the protein expression of Bax.Conclusion The four medicines inhibit proliferation of HepG2 and induce its apoptosis in vitro significantly.The effect of icaritin is the most significant.The mechanism may be related with the regulation of several Cyclins,Bax and Bcl-2.