3'-大豆苷元磺酸钠对大鼠脑缺血损伤后IL-10表达的影响

Effects of 3'-daidzein sulfonate on IL-10 Expression After Focal Cerebral Ischemia in Rats

目的:探讨3'-大豆苷元磺酸钠(DSS)对缺血再灌注损伤大鼠调节性T淋巴细胞因子IL-10表达的影响。方法:制作大鼠大脑中动脉栓塞脑缺血再灌注损伤模型,缺血后10 min,于舌下静脉给予1.0 mg·kg^(-1)和2.0 mg·kg^(-1)的DSS。缺血2 h,再灌注24 h后,TTC染色法测定脑梗死体积,干湿重法检测脑组织含水率,酶联免疫学方法测定血清及缺血侧脑组织血清白介素-10(IL-10)的含量,免疫组织化学及Western blot方法检测缺血半损伤区皮层组织IL-10蛋白的表达。结果:模型组大鼠出现明显的脑梗死灶,脑组织含水率升高,血清及脑组织IL-10含量降低;DSS治疗后,血清及脑组织IL-10含量升高。结论:DSS可通过提高调节性T淋巴细胞因子IL-10的表达,抑制脑缺血再灌注损伤后炎症反应。

Objective： To investigate the effect of 3 ＇-Daidzein Sulfonate Sodium（ DSS） on IL-10,an anti-inflammatory cytokine secreted by regulatory T cells,in cerebral ischemia / reperfusion injury. Method： A rat middle cerebral artery occlusion（ MCAO） model was established. 10 minutes after ischemia,1. 0 mg·kg^-1and 2. 0 mg·kg^-1DSS were given via the sublingual vein. After 2 h of ischemia and 24 h of reperfusion,brain water rate was calculated by the wet and dry weight method. Serum and brain tissue interleukin-10（ IL-10） content were examined by enzyme linked immunosorbent assay. The IL-10 protein expression in the ischemic penumbra region of cortical tissue was detected by immunohistochemistry and western blot assay. Result： DSS decreased cerebral infarct size and water content in rats with cerebral ischemia /reperfusion injury. It also significantly increased serum and brain tissue IL-10 content in the ischemic penumbra region.Conclusion： DSS can inhibit the inflammatory response after cerebral ischemia reperfusion injury through the increase of regulatory T cells factor,the expression of IL-10.