SALL4、OCT4、C-kit在精原细胞瘤中的表达观察

Expression of SALL4,OCT4 and C-kit in the Seminoma

目的:观察SALL4、OCT4、C-kit在精原细胞瘤中的表达,探讨其在该瘤病理诊断中的意义。方法:选取68例精原细胞瘤和20例病理形态相似的非精原细胞性肿瘤的术后标本,应用常规病理学技术及免疫组化Polink-2HRP染色系统检测SALL4、OCT4、C-kit的表达,并观察分析其与临床病理形态特征的关系。结果:SALL4、OCT4、C-kit在68例精原细胞瘤中的表达率分别为100%(68/68)、91.2%(62/68)、95.5%(65/68);在20例非精原细胞性肿瘤中的表达率分别为35%(7/20)、20%(4/20)、25%(5/20);SALL4、OCT4、C-kit在精原细胞瘤中的表达明显高于非精原细胞性肿瘤(P<0.05),差异有统计学意义。结论:SALL4在精原细胞瘤中的表达具有较高的敏感性,协同OCT4、C-kit使用,对其病理诊断具有重要价值。

Objective： To observe the expression of SALL4,OCT4 and C-kit in the seminoma,and to explore its significance in the diagnosis of the tumor. Methods： Postoperative samples of 68 seminoma and 20 non-seminoma with similar Pathologic morphology were selected. The expression of SALL4,OCT4 and C-kit were detected by routine pathological technique and immunohistochemical Polink-2HRP staining system. To observe and analyze the relationship between the clinical features and pathological characteristics. The relationship between the expressions and the clinical pathological morphological features was observed and analyzed. Results： The expression rates of SALL4,OCT4 and C-kit in 68 cases seminomas were 100%（ 68 /68）,91. 2%（ 62 /68） and 95. 5%（ 65 /68）,respectively. the expression rates in 20 cases of non-seminomas were 35%（ 7 /20）,20%（ 4 /20）,25%（ 5 /20）,respectively; The expression of SALL4,OCT4 and C-kit in the seminomas was significantly higher than that in the non-seminomas. The difference was statistically significant（ P〈0. 05）. Conclusion： The expression of SALL4 in the seminoma is highly sensitive. It is of great value in the pathological diagnosis if it is used with OCT4 and C-kit.