GC/MS/AMDIS在血中农药快速筛查中的应用研究

Study on rapid screening of pesticides in human blood by GC/MS/AMDIS

目的探索质谱自动解卷积技术（AMDIS）在血中农药快速筛查的应用,建立血中农药快速筛查的方法。方法采用乙酸乙酯提取全血中的农药,应用AMDIS及保留时间锁定（RTL）技术,快速筛查血中的农药。结果应用AMDIS及RTL技术可有效克服复杂基质干扰,建立了采用简单的样品前处理方法,快速筛查全血中的农药的GC/MS方法,16种常见农药的最小定性检出量为0.2~0.5μg。结论 AMDIS技术在复杂基质样品中的去干扰能力结合保留时间锁定技术,可快速准确筛查血中农药。

[Objective]To study the application of AMDIS for rapid screening of pesticides in human blood,establish a rapid screening method for pesticides in human blood.[Methods]The pesticides in human blood were extracted by ethyl acetate. AMDIS and Retention time locking（RTL） were used in rapid screening of pesticides in human blood.[Results] AMDIS and RTL technology could effectively overcome the complicated matrix interference,to establish a simple sample pretreatment GC/MS quick screening method for pesticide in human blood. The minimum qualitative detection limits of 16 kinds of pesticides were 0.2-0.5 μg.[Conclusion]AMDIS with anti-interference ability in complex matrix samples combined with retention time locking technology is a quick and reliable method for rapid screening of pesticides in human blood.