PD-1／PD-L1在三阴性乳腺癌中的表达及其意义

Expression of PD-1/PD-L1 in triple-negative breast carcinoma and its significance

目的：探讨程序性死亡分子1（PD-1）和程序性死亡配体1（PD-L1）在三阴性乳腺癌中的表达及其与临床病理特征的相关性。方法采用组织芯片结合免疫组织化学方法对151例三阴性乳腺癌（病例组）及65例非三阴性乳腺癌（对照组）进行染色，观察PD-1／PD-L1在各组的表达情况，分析其与各临床病理特征的关系。结果病例组肿瘤细胞和间质肿瘤浸润淋巴细胞中PD-1／PD-L1的表达均高于对照组，差异有统计学意义（ P ＜0.05）。其中，肿瘤细胞 PD-L1阳性率为16.6％（25／151），与高组织学级别、淋巴结转移相关（均P＜0.05）；间质肿瘤浸润淋巴细胞PD-L1阳性率为25.2％（38／151），与高组织学级别相关（P＜0.05）。间质肿瘤浸润淋巴细胞PD-1阳性率为27.2％（41／151），与间质肿瘤浸润淋巴细胞PD-L1表达呈正相关（P＜0.05）。结论 PD-L1在三阴性乳腺癌的表达与高组织学级别、淋巴结转移、间质肿瘤浸润淋巴细胞PD-1阳性相关。

Objective To investigate the correlation between the expression of PD-1, PD-L1 and clinicopathologic parameters in triple negative breast carcinoma （ TNBC ） .Methods Samples from 151 patients with TNBC and 65 cases of other breast carcinomas （ non-TNBC） were examined for PD-L1 and PD-1 expression by immunohistochemical staining on tissue microarray .Results The expression of PD-L1 and PD-1 in the tumor cells and interstitial lymphocytes in TNBC was significantly （ P〈0.05 ） higher than that in non-TNBC.In TNBC, the expression rates of PD-L1 in the cancer nests and stroma were 16.6%（25/151） and 25.2%（38/151） respectively.The former was positively correlated with tumor histological grade and lymph node metastasis （P〈0.05）, and the latter only with tumor histological grade （P〈0.05）. The expression rate of PD-1 was 27.2%（41/151）, and correlated with PD-L1 expression in stromal tumor-infiltrating lymphocytes （ P 〈0.05 ） .Conclusion The expression of PD-L1 in TNBC is significantly correlated with high-grade histology , lymph node metastasis and PD-L1 expression in stromal tumor-infiltrating lymphocytes .