茉莉酸合成抑制剂IBU对氯化镧调控玉米幼苗叶片抗盐性的影响

Effects of Jasmonic Acid Synthesis Inhibitor IBU on Salt Tolerance of Maize Seedling Leaves Regulated by Lanthanum Chloride

采用溶液培养的方法,研究茉莉酸合成抑制剂布洛芬(IBU)对氯化镧调控‘新单29’幼苗叶片抗盐性的影响。结果表明,与对照相比,盐胁迫显著提高玉米幼苗叶片的茉莉酸质量分数、MDA质量摩尔浓度、抗氧化酶SOD、CAT、POD、APX、DHAR活性、抗氧化物质GSH、AsA质量摩尔浓度及渗透调节物质可溶性糖和脯氨酸质量分数,最终使玉米幼苗单株生物量干质量显著下降。与单独盐胁迫相比,氯化镧使‘新单29’玉米幼苗叶片茉莉酸质量分数、抗氧化酶CAT、APX、GR、DHAR活性、抗氧化物质AsA质量摩尔浓度及渗透调节物质脯氨酸质量分数显著提高,从而使MDA质量摩尔浓度显著下降,最终导致玉米幼苗单株生物量干质量显著增加。与LaCl_3+盐处理相比,加入不同浓度的茉莉酸合成抑制剂IBU使玉米幼苗叶片茉莉酸质量分数、抗氧化酶SOD、CAT、APX、GR、DHAR活性、抗氧化物质AsA质量摩尔浓度及渗透调节物质脯氨酸质量分数均显著降低,从而使叶片MDA质量摩尔浓度显著增加,导致单株生物量干质量显著降低。这说明,茉莉酸参与氯化镧对玉米幼苗叶片抗盐性的调控。

Effects of jasmonic acid synthesis inhibitor IBU on salt tolerance of ＇Xindan 29＇ seedling leaf regulated by lanthanum chloride was studied. The results showed that salt stress significantly increased the molality of MDA, the activities of antioxidant enzymes SOD, CAT, POD, APX, DHAR, the molalities of antioxidant substances GSH and AsA and mass fractions of osmotic regulation substances soluble sugar and proline, and significantly reduced dry mass of plant compared with the control. Compared with salt stress, LaCl3 significantly increased mass fraction of jasmonic acid, the activities of CAT, APX, GR and DHAR, the molality of AsA and mass fraction of proline which resulted in the decrease of the molality of MDA increased in dry ass per plant. The experimental results further showed that adding different concentrations of jasmonic acid synthesis inhibitors IBU significantly decreased the mass fraction of jasmonic acid compared with LaCl3＋ salt treatment, the activities of SOD, CAT, APX, GR and DHAR, the molality of AsA and the mass fraction of proline resulted in the increase of the molality of MDA and the decrease of dry mass per plant. These results indicated that jasmonic acid involved in the salt resistance of maize seedling leaves was regulated by lanthanum chloride.