缬草黄酮和多糖的同步提取与分离工艺

Extracting flavonoids and polysaccharides synchronously from Valeriana officinalis L. and separating them

为探讨缬草黄酮与多糖的同步提取与分离工艺,以黄酮和多糖得率为评价指标,在单因素实验的基础上,利用响应面实验设计对提取工艺进行优化,采用萃取和醇沉技术分离黄酮和多糖。结果表明:在纤维素酶浓度1.9 U·mL^(-1)、料液质量浓度1∶28 g·mL^(-1)、提取温度49℃条件下,超声波辅助提取61 min,黄酮得率7.88%,纯度28.93%,多糖得率1.48%,纯度26.56%。优化后的工艺稳定可靠、提取得率较高,适合缬草黄酮与多糖的同步提取与分离。

To investigate the simultaneous extraction of flavonoids and polysaccharides from Valeriana officinalis L. and their subsequent separation,the yields of flavonoids and polysaccharides were used as major indexes.Based on single-factor experiments,the extraction process parameters were optimized by response surface analysis. Flavonoids and polysaccharides were separated by leaching and alcohol precipitation. The optimum extraction conditions were cellulase concentration of 1. 9 U·mL^-1,solid-to-liquid ratio of 1 ∶ 28 g·mL^-1,extraction temperature of 49 ℃,and ultrasonic treatment time of 61 min. Under the above conditions,the yields of flavonoids and polysaccharides reached 7. 88% and 1. 48%,respectively and their purities were 28. 93% and26. 56%. This optimized process,which is reliable and provides relatively high yields,is appropriate to extract flavonoids and polysaccharides synchronously from Valeriana officinalis L. and separate them.