十全育真汤对H_(22)荷瘤小鼠免疫功能的影响

Effects of Shiquanyuzhentang on immunologic function of H_(22) tumor-bearing mouse

目的:研究十全育真汤对荷瘤小鼠免疫功能的影响,探讨十全育真汤抗肿瘤的作用机制。方法:SPF级雄性昆明种小鼠30只,制成荷H_(22)小鼠肝癌细胞移植瘤模型,随机分为模型组、阳性对照组及十全育真汤组(n=10);另选择未接种小鼠10只为正常对照组。正常对照组、模型组每天按10 ml/kg灌胃生理盐水及蒸馏水,阳性对照组、十全育真汤组每天按8 g/kg、18 g/kg灌胃参一药液(80 mg/ml)与十全育真汤剂。连续给药14 d后处死小鼠,测量胸腺、脾脏指数及抑瘤率,检测外周血中白细胞、淋巴细胞含量及T细胞亚群CD3、CD4、CD8细胞百分比,血清中白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)及干扰素-β(IFN-β)的含量,淋巴细胞增殖能力及NK细胞杀伤功能。结果:较正常对照组,十全育真汤组小鼠体重明显增加,脾脏指数显著增大(P<0.05);白细胞、淋巴细胞CD4、CD8、CD3及TNF-α含量明显升高(P<0.05);IL-2、IFN-β含量显著下降(P<0.05);淋巴细胞增殖能力、NK细胞杀伤功能明显升高(P<0.05)。与模型组相比,十全育真汤组小鼠胸腺、脾脏指数显著增大(P<0.05);白细胞、淋巴细胞CD3、CD4、IL-2、IFN-β及TNF-α含量明显升高(P<0.05),CD8含量则显著降低(P<0.05);NK细胞杀伤功能及淋巴细胞增殖能力显著增加(P<0.05)。结论:十全育真汤可促进H_(22)荷瘤小鼠免疫器官的生长,增强机体免疫功能,有利于肿瘤机体的恢复。

Objective： To investigate the effects of Shiquanyuzhentang（ Traditional Chinese Medicine） on the immunologic function of tumor-bearing mice and its mechanism of antitumor. Methods： Thirty SPF grade male Kunming mice transplanted with H（22） hepatocellular carcinoma were divided into three groups randomly： model group,positive control group and Shiquanyuzhentang group（ n =10）. Another 10 mice were selected as normal control group. Normal control group and model group received normal saline and distilled water supplementation by 10 m L / kg everyday. Positive control group and Shiquanyuzhentang group received Shengyi（ 80 mg / ml）and Shiquanyuzhentang decoction at the doses of 8 g / kg and 18 g / kg respectively everyday. After 14 days of continuous administration,the mice were killed and the thymus,spleen index,tumor inhibition rate,peripheral blood leukocytes,lymphocyte content,cell percentage of T cell subsets CD3,CD4,CD8,interleukin 2（ IL-2） in serum,tumor necrosis factor-α（ TNF-α）,interferon β（ IFN-β） content,lymphocyte proliferation ability and NK cell were measured. Results： Compared with normal control group,the weight of mice and thymus and spleen index of the Shiquanyuzhentang group were increased significantly（ P〈0. 05）; leukocyte and lymphocyte CD3、CD4、CD8 and TNF-α were increased greatly（ P〈0. 05）,IL-2 and IFN-β were decreased significantly（ P〈0. 05）. Compared with model group,thymus and spleen index of Shiquanyuzhentang group were increased significantly（ P〈0. 05）; leukocyte and lymphocyte CD3、CD4、IL-2、IFN-β and TNF-α of Shiquanyuzhentang group were increased significantly（ P〈0. 05）,CD8 content was decreased significantly（ P〈0. 05）; NK cell and lymphocyte proliferation were increased significantly（ P〈0. 05）. Conclusion： Shiquanyuzhentang can promote the growth of immune organs in mice bearing H（22）,enhance immune function and is beneficial to the recovery of tumor body.