不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用研究

Impact of different temperatures and ambient PM2. 5 on the cytotoxicity and cytophagocytic function of alveolar macrophages of rat

目的:探究不同温度刺激与大气PM2.5对大鼠肺泡巨噬细胞的交互毒性作用。方法:提取大鼠肺泡巨噬细胞,分别在18℃、24℃、30℃、37℃、43℃下培养,每个温度组下设置不同浓度PM2.5处理,依次为100μg/ml、50μg/ml、25μg/ml、0μg/ml。处理8 h后,观察两因素对大鼠肺泡巨噬细胞组织形态学、细胞存活率和吞噬功能的影响。结果:同一温度下,不同剂量PM2.5组的相对细胞存活率和吞噬功能均显著低于空白对照组(P<0.05),且剂量越高,毒性作用越强,吞噬功能越弱。而在各温度组间,37℃组相对细胞存活率和吞噬功能最高,而随着温度的升高或降低,相对细胞存活率降低,吞噬功能下降。结论:不同温度刺激和大气PM2.5处理均会对大鼠肺泡巨噬细胞产生毒性作用,使其吞噬能力降低,并且温度与37℃差距越大、PM2.5浓度越高时毒性作用越明显。

Objective： To investigate the interactive effects of different temperatures and ambient PM2. 5 on the rat alveolar macrophages. Methods： The rat alveolar macrophages were collected. The cells were exposed in vitro to 18℃,24℃,30℃,37℃ and43℃ with PM2. 5 at the concentrations of 100 μg / ml,50 μg / ml,25 μg / ml and 0 μg / ml respectively. The cells were cultured in the different cases for 8 hours,then cytotoxicity was assessed by 3-（ 4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（ MTT） reduction assay and phagocytosis function of macrophages was assessed by neutral red absorption test. Results： The relative survival rate and the cytophagocytic function of alveolar macrophages of rats among the different concentration groups decreased significantly（ P〈0. 05） compared with the blank control group. Both were dose-dependent. The 37℃ group had the highest level of relative survival rate and the cytophagocytic function compared with other different temperatures groups. Interactive effect of different temperatures and ambient PM2. 5 was not observed. But the lower temperature and the higher PM2. 5 concentration group had stronger toxicity to alveolar macrophages. Conclusion： The results suggested that different temperatures and ambient PM2. 5 have cytotoxicity on alveolar macrophages,injuring the phagocytosis. The two factors had some interaction.