肺炎支原体感染后机体的体液免疫、外周血T淋巴细胞亚群含量的评估

Evaluation of the body's humoral immunity and peripheral blood T lymphocyte subset levels after mycoplasma pneumoniae infection

目的:评估肺炎支原体感染后机体的体液免疫、外周血T淋巴细胞亚群含量。方法:118例感染肺炎支原体的患儿作为观察组,同期在本院接受体检的健康儿童98例作为对照组,对比两组体液免疫、T淋巴细胞亚群、抗炎及促炎因子、Th1/Th2平衡状态等差异。结果:观察组血清IgA水平低于对照组(P<0.05),IgM水平高于对照组(P<0.05);外周血CD3^+、CD4^+T淋巴细胞水平及CD4^+/CD8^+比值低于对照组(P<0.05),CD8^+T淋巴细胞水平高于对照组(P<0.05);血清促炎因子白介素(IL)-8、肿瘤坏死因子-α(TNF-α)水平高于对照组(P<0.05),抗炎因子IL-10、IL-13水平低于对照组(P<0.05);血清IL-4、γ-干扰素(IFN-γ)、IL-4/IFN-γ水平高于对照组(P<0.05)。结论:肺炎支原体感染后患儿体液免疫及细胞免疫功能抑制,高炎症状态等均是导致疾病进展的重要机制。

Objective：To evaluate the body’s humoral immunity and peripheral blood T lymphocyte subset levels after mycoplasma pneumoniae infection.Methods：A total of 1 18 cases of children with mycoplasma pneumoniae infection were as observation group,and 98 cases of healthy children receiving medical examination in our hospital during the same period were as control group.The differences in humoral immunity,T lymphocyte subsets,anti-inflammatory and pro-inflammatory fac-tors,Th1/Th2 balance state,etc were compared between two groups of children.Results：Serum IgA level of observation group was lower than that of control group,and IgM level was higher than that of control group;peripheral blood CD3 ＋ and CD4 ＋ T lymphocyte levels as well as CD4 ＋/CD8 ＋ ratio were lower than those of control group,and CD8 ＋ T lymphocyte level was higher than that of control group;serum pro-inflammatory factors IL-8 and TNF-αlevels were higher than those of control group,and anti-inflammatory factors IL-10 and IL-13 levels were lower than those of control group;serum IL-4,IFN-γ and IL-4/IFN-γlevels were higher than those of control group.Conclusions：Humoral immune and cellular immune suppression, high inflammatory state and so on after mycoplasma pneumoniae infection are the important mechanisms leading to disease pro-gression.