宫颈癌及癌前病变组织中Twist及YB-1基因表达及与细胞侵袭的相关性

Twist and YB-1 gene expression in cervical cancer and precancerous tissue and their correlation with cell invasion

目的:研究宫颈癌及癌前病变组织中Twist及YB-1基因表达量与细胞侵袭的相关性。方法:收集2013年5月~2015年4月在我院手术切除的宫颈癌组织、癌前病变组织以及正常宫颈组织,采用免疫组化染色试剂盒测定Twist及YB-1基因的蛋白阳性表达率;采用荧光定量PCR试剂盒测定Twist、YB-1以及侵袭基因的mRNA表达量。结果:宫颈癌组织、癌前病变组织中Twist、YB-1的mRNA表达量和蛋白阳性表达率以及USP22、Rab11、Rac1、ANXA5的mRNA表达量均显著高于正常宫颈组织,宫颈癌组织中Twist、YB-1的mRNA表达量和蛋白阳性表达率以及USP22、Rab11、Rac1、ANXA5的mRNA表达量均显著高于癌前病变组织;Twist和YB-1阳性表达的宫颈癌及癌前病变组织中USP22、Rab11、Rac1、ANXA5的mRNA表达量显著高于Twist和YB-1阴性表达的宫颈癌及癌前病变组织。结论:宫颈癌及癌前病变组织中高表达的Twist及YB-1能够促进细胞侵袭。

Objective：To study the correlation of Twist and YB-1gene expression in cervical cancer and precancerous tissue with cell invasion.Methods：Cervical cancer tissue,precancerous tissue and normal cervical tissue surgically removed in our hospital between May 2013 and April 2015 were collected;immunohistochemical staining kits were used to detect the positive protein expression rate of Twist and YB-1gene;fluorescence quantitative PCR kits were used to detect Twist,YB-1and invasion gene mRNA expression.Results：Twist and YB-1 mRNA expression and positive protein expression rate as well as USP22,Rab11,Rac1 and ANXA5mRNA expression in cervical cancer tissue and precancerous tissue were significantly higher than those in normal cervical tissue,Twist and YB-1mRNA expression and positive protein expression rate as well as USP22,Rab11,Rac1 and ANXA5mRNA expression in cervical cancer tissue were significantly higher than those in precancerous tissue;USP22,Rab11,Rac1 and ANXA5mRNA expression in cervical cancer tissue and precancerous tissue with positive Twist and YB-1expression were significantly higher than those in cervical cancer tissue and precancerous tissue with negative Twist and YB-1expression.Conclusions：Highly expressed Twist and YB-1in cervical cancer and precancerous tissue can promote cell invasion.