子痫前期胎盘组织中骨桥蛋白表达量与细胞侵袭、血管新生的关系

Relationship between osteopontin expression and cell invasion as well as angiogenesis in preeclampsia placenta tissue

目的:研究子痫前期胎盘组织中骨桥蛋白(osteopontin,OPN)表达量与细胞侵袭、血管新生的关系。方法:选择2014年6月~2016年3月期间在我院确诊为子痫前期的56例患者作为子痫前期组(PE组),同期进行产检及分娩的80例正常孕产妇作为对照组。分娩后收集胎盘组织,采用免疫组化染色试剂盒测定OPN的表达量,采用酶联免疫吸附试剂盒测定细胞侵袭分子、血管新生分子的表达量。结果:PE组胎盘组织中OPN的阳性率显著低于对照组;PE组胎盘组织中细胞侵袭分子层黏连蛋白α4链(LAMA4)、尿激酶型纤溶酶原激活物(uPA)、Rho、Rho相关卷曲螺旋形成蛋白激酶(ROCK)以及血管新生分子血管内皮生长因子(VEGF)、胎盘生长因子(PIGF)、血管生成素相关生长因子(AGF)的蛋白表达量均显著低于对照组,OPN阳性胎盘组织中细胞侵袭分子LAMA4、uPA、Rho、ROCK以及血管新生分子VEGF、PIGF、AGF的蛋白表达量均显著高于OPN阴性胎盘组织。结论:子痫前期胎盘组织中OPN的低表达会对细胞侵袭分子、血管新生分子的表达产生抑制效应,进而抑制滋养细胞侵袭及胎盘组织中血管新生。

Objective：To study the relationship between osteopontin expression and cell invasion as well as angiogenesis in preeclampsia placenta tissue.Methods：A total of 56 patients diagnosed with preeclampsia in our hospital between June 2014 and March 2016 were selected as preeclampsia group（PE group）and 80 normal subjects receiving antenatal examination and giving birth during the same period were selected as control group.After labor,the placenta tissue was collected,immunohistochemical staining kits were used to detect OPN expression and enzyme-linked immunosorbent assay kits were used to determine cell invasion and angiogenesis molecule expression.Results：Positive OPN rate in placenta tissue of PE group was significantly lower than that of control group;cell invasion molecules LAMA4,uPA,Rho and ROCK as well as angiogenesis molecules VEGF,PIGF and AGF protein expression in placenta tissue of PE group were significantly lower than those of control group,and cell invasion molecules LAMA4,uPA,Rho and ROCK as well as angiogenesis molecules VEGF,PIGF and AGFprotein expression in OPN-positive placenta tissue were significantly higher than those in OPN-negative placenta tissue.Conclusions：Lowly expressed OPN in preeclampsia placenta tissue will inhibit the expression of cell invasion molecules and angiogenesis molecules so as to inhibit sertoli cell invasion and the angiogenesis in placenta tissue.