母体铅暴露对仔鼠大脑皮层组织中IL-1β和TNF-α表达的影响

Effects of early life lead exposure on expression of IL-1β,TNF-α in cerebral cortex of mouse pups

探讨母体铅染毒对其仔鼠大脑皮层组织中IL-1β和TNF-α表达量的影响,揭示铅神经毒性的潜在机制。母鼠采用自由饮水的方式自妊娠1d开始经饮水染铅(0.1%,0.5%和1.0%的浓度溶解在去离子水中,对照组饮蒸馏水)至仔鼠出生后21d断乳为止,每组10只。于出生后21d,用石墨炉原子吸收光谱仪测定仔鼠血液和大脑皮层组织中铅含量。用免疫组织化学染色法和Western blot方法测定大脑皮层组织中IL-1β和TNF-α蛋白表达情况。结果表明,各剂量染铅组血铅和脑铅含量与对照组比较明显升高(P<0.05);与对照组相比,随着试验组染铅浓度的升高,IL-1β和TNF-α的表达量显著增加(P<0.05)。铅可能通过诱导大脑皮层中IL-1β和TNF-α的高表达,影响学习记忆从而造成神经系统损害。

To investigate the effects of maternal lead （Pb） exposure on the expression of interleu- kin1-β（IL-1β） ,tumor necrosis factor （TNF-α） in cerebral cortex of mice offspring and on learning and memory ability. Pb was exposed to mice from beginning of gestation to weaning at 21 d with Pb acetate administered in drinking water at the concentrations of 0.1%,0.5% and 1.0% groups, respectively. On the PND21 ,the Pb levels of blood and cerebral cortex were determined by graphite furnace atomic absorption spectrometry. The expression of IL-1β,TNF-α in cerebral cortex was measured by immunohistochemistry and Western blot. The Pb level in blood and cerebral cortex of all exposure groups was significantly higher than that of the control group （P〈0.05）. The expression of IL-1β,TNF-α was higher in Pb exposed groups than that of the control group （P〈0. 05）. The high expression of IL-1β,TNF-α in the cerebral cortex of pups may contribute to the impairment of learning and memory,which is associated with maternal Pb exposure.