芝麻油对大鼠脑缺血再灌注损伤的保护作用及其机制

Protective Effect of Sesame Oil Against Brain Ischemia Reperfusion Injury on Rats and Related Mechanism

目的:探讨芝麻油对大脑缺血再灌注损伤的保护作用及其作用的机制。方法:实验将雄性SD大鼠20只随机分为两组,对照组(C组,n=10),行大脑中动脉阻断前5 d及术前1 h予生理盐水4 m L/(kg·d)灌胃;芝麻油组(S组,n=10)于中动脉阻断前5 d及术前1 h给予芝麻油4 m L/(kg·d)灌胃。脑缺血90 min后恢复血流,于大脑中动脉阻断后24 h处死大鼠,测各组脑组织中核转录因子(Nuclear factor kappa B,NF-κB)P65表达阳性率(%)、丙二醛(malonaldehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)的水平;同时检测C组和S组的脑水肿指数、脑梗死的体积、神经功能评分。结果:脑梗死S组大鼠神经行为学评分明显低于C组,S组大鼠缺血侧的大脑水肿指数、脑梗死体积较C组明显改善(P<0.01):同时S组与C组比较,脑组织中核转录因子P65表达阳性率[(20.91±1.36)%vs(27.13±3.57)%]、MDA含量[(13.70±2.54)nmol/mg vs(3.96±0.68)nmo L/mg]均降低,SOD活性提高[(272.6±23.75)U/mg vs(189.5±16.53)U/mg],差异均有统计学意义(P<0.05)。结论:脑梗死期应用芝麻油可减轻脑组织的氧化应激反应,抑制炎症因子释放,从而对局灶性脑缺血后脑损伤具有保护作用。

Objective：To investigate the effect of sesame oil on the focal brain ischemia reperfusion injury and the related mechanism.Method：Twenty Sprague Dawley male rats were randomly divided into two groups：the control group was given middle cerebral artery occlusion（MCAO） and saline pretreatment for 5 days as placebo（n=10）.Sesame oil group was given sesame oil via gastric gavage at a dose of 4 m L/（kg·d） for 7 days（n=10）.Middle cerebral artery was occluded by thread for 90 min,followed by reperfusion.The neurological outcomes including edema index,infarc size and neurological deficit scores were evaluated 24 h after MCAO.The levels of malonaldehyde（MDA）,the expression change of NF-κB/P65 and activity of superoxide dismutase（SOD） in brain tissue were assessed according to the corresponding kits.Result：Compared with C group,sesame oil significantly reduced the edema index,infarc size and neurological deficit scores at 24 h after MCAO（P〈0.01）.Also,sesame oil decreased the tissue levels of the expression change of NF-κB/P65 [（20.91±1.36）% vs（28.45±3.57）% ],and MDA[（13.70±2.54）nmol/mg vs（3.96±0.68）nmol/mg,P〈0.05],and enhanced the activity of SOD[（272.6±23.75）U/mg vs（189.50±16.53）U/mg,P〈0.05] compared to C group.Conclusion：Sesame oil can protect against focal brainischemia reperfusion injury partly through the mechanism of attenuating oxidative stress and inhibiting the proinflammatory response.