乙型肝炎患者病毒复制指标与乙肝标志物及肝功能关系分析

The relationship between HBVDNA and HBV markers,liver function on Hepatitis B patients

目的探讨乙型肝炎患者病毒复制指标(HBV DNA)与乙肝标志物及肝功能的关系。方法对68例乙型肝炎患者采用电化学发光法检测血清中的乙型肝炎e抗体(HBe Ab)、核心抗体(HBc Ab)、e抗原(HBe Ag)、表面抗体(HBs Ab)、表面抗原(HBs Ag)水平。采用实时荧光定量法检测血清中的HBV DNA水平,采用全自动生化仪检测血清中的天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)和胆碱酯酶(CHE)。结果 HBs Ag/HBe Ag/HBc Ab阳性组(大三阳组)患者血清中的HBV DNA阳性率以及定量结果高于其他组(P<0.05);HBe Ag阳性组中HBVDNA的阳性表达率高于HBe Ag阴性组中HBV DNA的阳性表达率(P<0.05);HBV DNA载量≤10~3、10~3<HBV DNA载量<106、HBV DNA载量≥106的患者间血清AST和ALT水平差异有统计学意义(P<0.05),随着HBVDNA载量的增加血清AST和ALT显著增加;但三组间的CHE差异无统计学意义(P>0.05)。结论乙型肝炎患者HBV DNA水平和乙肝患者的病毒复制情况、炎症程度有着密切的联系,但需要与乙肝标志物结合才能更加客观和准确的评价患者的病情变化和严重程度。

Objective To explore the relationship between HBV DNA and HBV markers,liver function on Hepatitis B patients.Methods Serum HBe Ab,HBc Ab,HBe Ag,HBs Ab and HBs Ag were detected by electrochemiluminescence immunoassay（ ECLIA）.HBV DNA in serum and was detected by real-time fluorescence quantitative.serum AST,ALT and CHE were detected by automatic biochemical analyzer.Results HBV DNA positive rate and quantification of serum in HBe Ag patients were Significantly higher than the other groups（ P〈0.05）.HBVDNA positive expression rates of HBe Ag-positive group was significantly higher than that in HBe Ag-negative group of positive expression of HBV DNA（ P〈0.05）.In serum HBV DNA≤10^3,10^3 〈HBV DNA 〈10^6,have serum AST and ALT levels between patients HBV DNA load≥106significant difference（ P〈0.05）.Conclusion Hepatitis B patients HBV DNA levels and virus replication,the degree of inflammation in patients with hepatitis B are closely linked,the severity and the condition and needs HBV DNA and HBV markers were evaluated in combination.