卷烟主流烟气烟熏香成分的感官导向分析

Sensory-oriented analysis of smoky components in mainstream cigarette smoke

以感官导向分析方法研究了卷烟主流烟气总粒相物中的烟熏香成分。利用凝胶渗透色谱(GPC)分离卷烟主流烟气粒相物,评价了各分离流份的香气特征,并定位烟熏香特征流份;通过GC/MS法定性分析结合标准物质香气特征评价,鉴定出26种烟熏香成分;建立了主流烟气粒相物中26种烟熏香成分的GC/MS定量分析方法,测定了其在9个品牌卷烟主流烟气中的释放量及在烟气粒相物中的质量分数;利用三点选配法(3-AFC)测定了26种烟熏香成分在乙醇和三乙酸甘油酯中的嗅觉阈值,结合其在卷烟烟气粒相物中的质量分数,计算了9个品牌卷烟烟气粒相物中各烟熏香成分的香气活性值(OAV),推测了26种烟熏香成分的贡献度。结果表明:1在所考察卷烟的主流烟气中,释放量较大的烟熏香成分均为苯酚、对甲酚、4-乙烯基愈创木酚、邻甲酚、2,4-二甲基苯酚和2-(4-羟基苯基)乙醇。2愈创木酚、对甲酚和5-甲基愈创木酚对卷烟烟熏香韵的贡献最大。

A sensory-oriented analysis was carried out to investigate the smoky components in total particulate matter （TPM） of mainstream cigarette smoke. The TPM of mainstream cigarette smoke was separated by gel permeation chromatography （GPC）, the aroma characteristics of each separated fraction were estimated, and the fractions with characteristic smoky aroma were located. A total of 26 smoky components were identified by qualitative GC/MS analysis combined with aroma evaluation of standard substances, and a quantitative GC/MS method was developed for analyzing the 26 smoky components in TPM of mainstream cigarette smoke. The release of the 26 smoky components in mainstream smoke from cigarettes of 9 brands and their mass fractions in TPMs were determined. The olfactory thresholds of the 26 smoky components in ethanol and triacetin were determined by three-alternative forced-choice （3-AFC） method, and their odor activity values （OAVs） in TPMs of cigarettes of 9 brands were calculated on the basis of their mass fractions, then their contributions to the smoky aroma of cigarette smoke were estimated. The results showed that： 1） In the mainstream smoke of the tested cigarettes, the release of phenol, 4-methylphenol, 4-vinylguaiacol, 2-methylphenol, 2,4-dimethylphenol and tyrosol was higher than the other smoky components. 2） The contributions of guaiacol, 4-methylphenol and 5-methylguaiacol to the smoky aroma of cigarette were the most significant based on their OAVs.