大豆抗感资源对大豆胞囊线虫3号生理小种生长发育动态的影响

The Influence of Resistant and Susceptible Soybean Germplasm on Growth Dynamic Changes of Soybean Cyst Nematode Race 3 (SCN3)

采用苗期盆栽的方法,以五寨黑豆(抗)、07-2-10-6(抗)和合丰35(感)、07-2-7-14(感)为试验材料,测定不同抗性大豆对胞囊线虫3号生理小种生长发育动态的影响。结果表明,五寨黑豆及07-2-10-6根系分泌物能抑制线虫卵的孵化,而合丰35及07-2-7-14根系分泌物能刺激线虫卵的孵化。培养10d后,合丰35及感病后代07-2-7-14的线虫卵孵化相对值分别为3.71和4.38,五寨黑豆及抗病后代07-2-10-6的线虫卵孵化相对值分别为0.89和1.39。大豆根内各龄线虫数量的动态变化结果表明,侵入抗病品种中的J2幼虫数量较少,只有部分J2幼虫可以进一步发育成高龄期的线虫,且发育速度迟缓,雌雄比值为1∶4.13;侵入感病品种中的J2幼虫群体数量大,多数能够正常发育成更高一级的虫体,发育速度明显大于抗病品种中的J2幼虫,雌雄比值为10.56∶1。五寨黑豆抗大豆胞囊线虫是其根外抗性、抗发育和抗繁殖多种抗病机制综合作用的结果。

A pot experiment was conducted with resistant cultivars Wuzhai Heidou, 07-2-10-6 and susceptible eultivars Hefeng 35, 07-2-7-14. The influence of different germplasm to growth dynamic changes of soybean cyst nematode race 3 （ SCN3 ） were determined. The results indicated that root exudates had an important effect on the egg hatch of SCN3. Root exudates of Hefeng 35 and 07-2-7-14 stimulated egg incubation of SCN3, while root exudates of Wuzhai Heidou and 07-2-10-6 inhibited egg incubation of SCN 3. After incubating 10 days, the J2 hatching rate of Hefeng 35, 07-2-7-14 were 3.71 and 4.38, respectively, while the J2 hatching rate of Wuzhai Heidou, 07-2-10-6 were 0.89 and 1.39, respectively. The dynamic changes of SCN3 in soybean root indicated that the number of J2 seldom invaded into resistant cultivars. There was only a part of larva at J2 stage that could develop to J3 stage, and their growth was delayed that the ratio of female to male was 1：4.13. But there was a large number of J2 stage larva that invaded into susceptible cultivars, and most of them could develop to J3 stage. The growth speed of larva in susceptible cultivars was faster than that in resistant cultivars, and the ratio of female to male was 10.56：1. The resistant mechanism ofWuzhai Heidou to SCN 3 was integrated effects of anti-intrusion, anti-growth and anti-reproduction.