维药降糖孜亚比提片定性定量方法研究

Study on Qualitative and Quantitative Methods of Jiangtangziyabiti Tablets

目的:建立维药降糖孜亚比提片的定性定量方法。方法:采用薄层色谱法对鹿茸、人参和石榴皮进行薄层鉴别。采用高效液相色谱法对人参中特征成分人参皂苷Rg_1、人参皂苷Re和人参皂苷Rb_1进行含量测定。结果:薄层色谱中,定性鉴别斑点清晰,且阴性无干扰,方法稳定可靠。人参皂苷Rg_1对照品在0.0151~0.4840 mg/mL范围内,线性关系良好(r=0.9996),平均回收率(n=6)为96.20%,RSD为1.58%;人参皂苷Re对照品在0.0584~0.9340 mg/mL范围内,线性关系良好(r=1),平均回收率(n=6)为102.14%,RSD为1.64%;人参皂苷Rb_1对照品在0.0689~1.1020mg/mL范围内,线性关系良好(r=1),平均回收率(n=6)为97.15%,RSD为1.36%。结论:采用该方法专属性好,灵敏度高,结果稳定可靠,可用于维药降糖孜亚比提片的质量控制。

Objective To establish the qualitative and quantitative methods of Jiangtangziyabiti tablets.Methods Cervi Cornu Pan-totrichum,Ginseng Radix et Rhizoma and Granati Pericarpium were identified by TLC.To establish the HPLC methods to determine the content of three ginsenosides：Rg1 ,Re and Rb1 in Jiangtangziyabiti tablets.Results Identification spots were clear,and this meth-ods was stable and reliable.The ginsenoside Rg1 control in 0. 0151 -0. 4840 mg/mL range,good linear relationship,r=0. 9996,av-erage recovery=96. 20%,RSD=1. 58%;ginsenoside Re control in 0. 0584 -0. 9340 mg/mL range,good linear relationship,r=1, the average recovery rate-102. 41%,RSD=1. 64%. ginsenoside Rb1 control in 0. 0689 -1. 1020 mg/mL range,good linear rela-tionship,r=1,the average recovery rate=97. 15%,RSD=1. 36%. Conclusion This method is simple,reliable,stable and can con-trol the quality of ginseng in compound preparation.