人食管癌间充质干细胞对食管癌EC109细胞增殖的影响

Effects of human esophageal cancer mesenchymal stem cells on the proliferation and cell cycle of EC109 cells

背景:研究表明肿瘤细胞释放的生长因子及细胞因子等生物信号可将间充质干细胞迁移至肿瘤组织,进而使间充质干细胞分化形成微环境中的各种基质细胞,参与调节肿瘤细胞的生长。目的:探讨人食管癌组织间充质干细胞对食管癌EC109细胞增殖和细胞周期的影响。方法:从食管癌组织中分离和纯化出食管癌间充质干细胞,以不同数量(1×10~4,2×10~4,3×10~4,4×10~4个)与食管癌EC109细胞系共培养,采用MTT法检测人食管癌组织间充质干细胞对EC109细胞增殖的影响;流式细胞仪检测人食管癌组织间充质干细胞对EC109细胞周期的影响。结果与结论:(1)随着人食管癌组织间充质干细胞数量增加和作用时间(24,48,72 h)的延长,人食管癌组织间充质干细胞对食管癌细胞株EC-109有抑制作用,细胞存活率明显下降,差异有显著性意义(P<0.05);(2)随着人食管癌组织间充质干细胞数量的增加,食管癌细胞株EC-109 G_1期细胞比例显著增高,G_2期细胞比例显著降低;(3)结果表明,人食管癌组织间充质干细胞能抑制EC109细胞的增殖且改变细胞周期的分布,并呈一定的剂量和时间依赖性。

BACKGROUND： Studies have shown that biological signals such as growth factors and cytokines released from tumor cells can make mesenchymal stem cells migrate into tumor tissues, and then these cells differentiate into various stromal cells constituting a microenvironment to participate in the regulation of tumor cells. OBJECTIVE： To investigate the effects of human esophageal cancer mesenchymal stem cells （hEC-MSCs） on the proliferation and cell cycle of human esophageal carcinoma cell line EC109. METHODS： After isolation and purification, hEC-MSCs at 1×10^4, 2×10^4, 3×10^4, 4× 10^4 were co-cultured with EC109 cells, respectively. MTT method and flow cytometry were used to measure cell proliferation and cell cycle of EC 109 cells, respectively. RESULTS AND CONCLUSION： With the increased number from 1 ~104 to 4x104 and increased exposure time from 24 to 72 hours, hEC-MSCs inhibited the proliferation of EC109 cells, and the survival rate of EC109cells was decreased significantly （P 〈 0.05）. Cell cycle analysis revealed a marked increase in the EC-109 G1 phase and a decrease in the EC-109 G2 phase in a number-dependent manner. To conclude, hEC-MSCs inhibit the proliferation of EC109 cells and vary the cell cycle in number- and time-dependent manners.