生长因子复合支架材料在即刻种植周围骨缺损中的作用

Growth factor composite scaffolds for bone defect repair via immediate implantation of bone defects

背景:研究发现神经生长因子在骨的愈合过程中发挥重要作用,但关于生长因子复合支架材料在犬即刻种植周围骨缺损中作用的研究不多。目的:分析神经生长因子复合支架材料在犬即刻种植周围骨缺损中的作用。方法:制备神经生长因子复合锶磷灰石支架材料,建立犬下颌骨缺损动物模型,将犬下颌骨缺损种植体分为实验组、阳性对照组和空白对照组,实验组植入神经生长因子复合锶磷灰石;阳性对照组植入锶磷灰石;空白对照组不植入任何材料。观察3组犬下颌骨种植体周围骨缺损的CT三维图像重建情况和苏木精-伊红染色情况。结果与结论:1空白对照组犬骨缺损中央没有新骨生成,周围有少量新生骨生成;阳性对照组和实验组犬骨缺损处骨小梁从中间向四周扩散形成骨修复;2实验组犬下颌骨种植体周围骨小梁的骨密度、数量、厚度、种植体-骨结合率均高于阳性对照组和空白对照组(P<0.05),阳性对照组4项指标高于空白对照组(P<0.05);实验组犬下颌骨种植体周围骨小梁分离度低于阳性对照组和空白对照组(P<0.05),阳性对照组低于空白对照组(P<0.05);3苏木精-伊红染色显示,实验组犬下颌骨种植体支架网孔中有大量新骨生成,骨小梁排列整齐,周围骨质和新生组织连接紧密;阳性对照组犬下颌骨可见新生类骨质和骨小梁,以及少量死骨碎片;空白对照组骨缺损处有部分新骨生成,骨小梁排列不整齐,周围骨质和新生组织连接不紧密;4结果表明,神经生长因子复合支架材料能够促进犬即刻种植周围骨缺损的骨生成,是一种比较好的骨修复材料。

BACKGROUND： Nerve growth factor has been shown to play an important role in bone healing, but little is reported on the effect of growth factor composite scaffolds via the immediate implantation in the repair of canine bone defects. OBJECTIVE： To analyze the effect of nerve growth factor composite scaffolds via the immediate implantation for the repair of canine bone defects.METHODS： Nerve growth factor composited strontium apatite scaffolds were prepared. Canine mandibular defect models were established and divided into three groups, followed by implanted with composite scaffold（experimental group）, strontium apatite（positive control group）, or nothing（blank control group）. The three-dimensional CT reconstruction and hematoxylin-eosin staining of canine mandibular bone defects were observed. RESULTS AND CONCLUSION： In the blank control group, there were few new bones surrounding bone defect. Trabecular bones spread from the defect center to the surrounding tissues in the experimental and positive control groups. The bone density, volume, thickness, and implant-bone contact were significantly increased, while the trabecular separation was significantly decreased in the experimental group compared with the positive control and blank control groups（P〈0.05）, and all above indicators in the positive contro group were significantly higher than those in the blank control group（P〈0.05）. Hematoxylin-eosin staining showed that in the experimental group, there were a large number of new bones that contacted with the surrounding bones closely, and trabecular bones arranged regularly. In the positive control group, newborn osteoid, trabeculare, and a small amount of debris were found. In the blank control group, few new bones were connected with the surrounding bones untightly and trabecular bone arranged irregularly. These results indicate that the nerve growth factor composite scaffold can promote the bone regeneration in the canine bone defects after immediate implantation.