载细胞甲壳素曲率混合膜修复角膜上皮损伤

Chitin hybrid membrane carrying cells repairs corneal epithelial injury

背景:研究发现甲壳素是一种比较好的生物材料,但关于甲壳素载角膜上皮细胞混合膜修复兔角膜上皮损伤的研究不多。目的:观察载角膜上皮细胞甲壳素曲率混合膜对兔角膜上皮损伤的修复作用。方法:取18只新西兰大白兔,建立左眼角膜上皮损伤模型,随机分2组,实验组于角膜上皮损伤处植入载角膜上皮细胞甲壳素曲率混合膜,对照组植入甲壳素曲率混合膜。植入后1,3,7 d,观察角膜上皮损伤面积、眼角膜病理组织学及超微结构。结果与结论:1角膜上皮损伤面积:实验组植入后1,3 d的角膜损伤面积明显小于对照组(P<0.05),两组植入后7 d的角膜损伤均全完愈合;2眼角膜病理组织学观察结果:植入后7 d,实验组角膜上皮层和基质层连接紧密,有6层左右上皮细胞层,修复完整,结构整齐,最外层平整光滑;对照组角膜上皮层也和基质层连接紧密,有6层左右上皮细胞层,修复完整,但上皮层层次紊乱,最外层表面不平整;3眼角膜超微结构:植入后7 d,实验组角膜上皮细胞细胞为六边形,排列较紧密,细胞表面平整;对照组角膜上皮细胞也为六边形,表面不平整,细胞之间有间隙;4结果表明:载角膜上皮细胞甲壳素曲率混合膜可促进大白兔角膜上皮损伤的修复。

BACKGROUND： Chitin has been found to be a good biomaterial, but research on chitin carrying corneal epithelial cells for rabbit corneal epithelial injury is little reported.OBJECTIVE： To investigate the repair outcomes of chitin hybrid membrane carrying corneal epithelial cells in the rabbit corneal epithelial injury.METHODS： Eighteen New Zealand white rabbits were enrolled and made into left corneal epithelial injury models, and then randomized into two groups and treated with chitin hybrid membrane carrying corneal epithelial cells（experimental group） and chitin hybrid membrane（control group）, respectively. The damage area, histological changes and ultrastructure of the cornea were observed at 1, 3, and 7 days after implantation. RESULTS AND CONCLUSION： Damage area of the cornea in the experimental group was significantly less than that in the control group at 1 and 3 days after implantation（P〈0.05）, and the cornea in both two groups healed well at 7 days after implantation. At 7 days after implantation, in both two groups, the corneal epithelium with six layers adhered to the corneal stroma closely, which was repaired completely and regularly. Comparatively speaking, the cornea in the experimental group possessed smooth outer layer. Besides, in the experimental group, the hexagonal corneal epithelial cells arranged closely with flat surface; while the hexagonal corneal epithelial cells in the control group showed no smooth surface and gaps between cells. These results indicate that chitin hybrid membrane carrying corneal epithelial cells promotes the repair of rabbit corneal epithelial injury.