内蒙古呼和浩特地区丙型肝炎病毒基因型分布

Genotypes of the Hepatitis C Virus in Infected Patients in Hohhot,China

为了解内蒙古呼和浩特地区丙型肝炎病毒(HCV)基因型的分布特征,为本地区丙型肝炎的治疗、预防提供基础数据资料。收集呼和浩特地区2014年1月至2015年1月就诊的门诊和住院丙型肝炎患者血清采样标本149份,均为HCV抗体检测阳性且HCVRNA定量检测阳性。QIAGEN柱式病毒RNA提取法提取HCV RNA,反转录成cDNA,巢式PCR法扩增HCVNS5B区,对扩增片段进行测序,在NCBI BLAST上进行序列比对得到相似度最大的参考序列并确定基因型,用Megalign,Clustal W进行序列分析并建立同源关系树,分析呼和浩特地区HCV感染基因型的分布特征,以及基因型与宿主性别、年龄的关系。在测序成功的94份样本中,共检出6个基因型,1b型占73.40%(69/94),2a型占19.14%(18/94),3a、3b、6a型各占2.12%(2/94),6u型占1.06%(1/94)。性别资料明确的93例样本,男女基因型分布无显著差异(P>0.05)。年龄资料明确的90例HCV样本,1+2型的患病年龄高于3+6型的患病年龄有统计学意义(P<0.05)。得出内蒙古呼和浩特地区HCV感染的基因型1b为主,其次为2a型,但也有3型、6型等基因型的传入。HCV的基因型4型和5型未检测到。

We wished to identify the existing HCV genotypes in diagnosed cases of infection by the hepatitis C virus in Hohhot （China） to offer basic data for the treatment and prevention of HCV infection in this area. Outpatients and inpatients were recruited from hospitals in Hohhot from January 2014 to January 2015. In total, 149 patients with HCV infection confirmed by positive anti-HCV and HCV-RNA tests were selected. First, we extracted HCV RNA. cDNA was obtained using reverse transcription, then NS5B regions were amplified using a nested polymerase chain reaction （PCR）, which enabled 94 cases to be sequenced. We compared sequences in NCBI BLAST, which revealed the reference sequence of maximum similarity and enabled identification of HCV genotypes. Then, a homologous relationship tree was created using MegAlign clustal W. Finally, the distribution characteristics in HCV genotypes, as well as the relationship between genotypes and host age and sex, were obtained. Genotype lb accounted for 73. 40% （69/94）, 2a for19.14% （18/94）, 3a, 3b, and 6a for 2.12% （2/94）, respectively, and 6uforl. 06% （1/94）. The genotype distribution of men and women showed no significant difference （P〉0.05） in 93 cases for whom explicit sex-specific data were available. Age data for 90 patients revealed the age of disease onset of the land2 types to be significantly higher than that of the 3and6 types （P〈0. 05）. Hence, genotype lb was the most prevalent in Hohhot （lb has been reported to be the predominant genotype in the general population of China, followed by genotype 2a）. Other, less prevalent genotypes were 3a, 3b, 6a and 6u. Genotype 4 and genotype 5 were not found.