过继转移日本血吸虫感染鼠DC调控IL-13抑制过敏性哮喘黏液分泌的机制研究

Adoptive transfer of DCs from mice infected with Schistosoma japonicum inhibits mucus secretion of OVA-induced allergic asthma by regulating IL-13 production

目的探讨过继转移日本血吸虫感染鼠DC对IL-13细胞因子调控抑制过敏性哮喘黏液分泌的作用机制。方法采用MACS磁珠分选方法从日本血吸虫(SJ)感染小鼠及健康对照小鼠脾脏中提取DC,分别过继转移给BALB/c小鼠,并用OVA诱发哮喘。4周后处死小鼠,提取肺组织总RNA并制作病理切片,采取qRT-PCR和PAS染色方法等检测IL-13mRNA以及蛋白水平。建立DC与CD4+T细胞共培养体系,检测IL-13的表达情况。结果肺组织病理学检查显示,与OVA单纯哮喘组比较,过继转移SJ感染DC组(SJ+OVA)过敏性哮喘被抑制。qRT-PCR显示,过继转移SJ感染DC组IL-13mRNA表达受到抑制。PAS染色显示SJ感染DC组黏液分泌减少,免疫组化检查该组IL-13的表达量降低。DC与CD4+T细胞共培养结果显示SEA处理DC能抑制CD4+T细胞IL-13的表达。结论过继转移日本血吸虫感染鼠DC通过下调CD4+T细胞中IL-13细胞因子的释放而抑制过敏性哮喘的黏液分泌。

Objective To examine the inhibitory effect of dendritic cells（DC）from mice infected with Schistosoma japonicum on allergic asthma and their regulation of IL-13 production. Methods DCs were isolated from the spleen of mice infected with S.japonicumusing MACS and were adoptively transferred to BALB/c mice.Allergic asthma was then induced with OVA.Normal mice and untreated mice with induced asthma served as controls.Four weeks later,the lungs were removed from the groups of mice.Total RNA was extracted for qRT-PCR and sections were prepared for histological analysis.DCs and CD4^＋T cells were co-cultured in vitro,and IL-13 mRNA was detected using qRT-PCR. ResultsThe DCs＋ OVA group had markedly inhibited lung inflammatory response compared to the control（OVA vs.DCs＋OVA：IL-13,P 0.05;MUC5AC,P〈0.01）.Levels of both mRNA and protein also decreased markedly in the DCs＋OVA group.In an in vitro experiment,DCstreated with SEA inhibited IL-13 production by CD4^＋T cells（untreated group vs.group treated with SEA：48h,P〈0.01;72h,P〈0.05）. Conclusion Adoptive transfer of DCsfrom mice with schistosomiasis inhibited mucus secretion in OVA-induced allergic asthma by regulating IL-13 production.