饲料浮萍水平对大正三色锦鲤抗氧化能力、免疫力及消化酶活性的影响

Effects of different levels of duckweed on antioxidant ability,digestive ability and immunity of Tajsho-sanke

为探究饲料中不同浮萍添加水平对大正三色锦鲤抗氧化能力、免疫能力以及消化能力的影响。配制浮萍添加水平分别为0%(对照组)、3%、6%、9%、13%、14%的6种等蛋白(38.6%)饲料,饲养初重为(42.19±1.32)g大正三色锦鲤8周,分别记为Diet 1(0/13)、Diet 2(3/10)、Diet 3(6/7)、Diet 4(9/4)、Diet 5(13/1)、及Diet 6(14/0)6组。每种饲料设3个重复,每个重复饲喂10尾鱼。结果表明:(1)饲料中浮萍替代菜粕的量在13%时,大正三色锦鲤的抗氧化能力最强,肝脏中超氧化物歧化酶(SOD)与过氧化氢酶(CAT)活力较对照组提高41.99%、20.85%(P<0.05)。(2)Diet 5(13/1)组锦鲤的免疫能力提高并达到最佳状态,血清中谷丙转氨酶(GPT)与碱性磷酸酶(AKP)较对照组提高66.61%、73.27%(P<0.05)。(3)浮萍添加量在13%时,大正三色锦鲤各项消化酶活性达到最佳,前肠蛋白酶活性与脂肪酶活性较对照组提高了38.18%、58.22%(P<0.05)。因此,建议在饲料中添加13%的浮萍。

This experiment was conducted to investigate the effects of dietary duckweed levels（0%,3%,6%,9%,13% and 14%,named as Diet 1（0/13）,Diet 2（3/10）,Diet 3（6/7）,Diet 4（9/4）,Diet 5（13/1）and Diet 6（14/0）respectively）on the antioxidant ability,digestive ability and immunity in Tajsho-sanke.A total of 180 average weight of（42.19±1.32）g Tajsho-sanke were randomly allocated to 6 groups with 3 replicates of 10 each.The results showed as follows：（1）compared with the control group,superoxide dismutase（SOD）and catalase（CAT）of hepatopancreas of fish fed with Diet 5（13/1）were increased by 41.99 and 20.85% respectively（P 0.05）.（2）the highest values of glutamic-pyruvic transaminase（GPT）and alkaline phosphatase（AKP）activities of serum were observed in the Diet 5（13/1）group and they were 66.61%and 73.27% higher than the control group（P 0.05）.（3）the activities of protease and lipase of foregut in the Diet 5（13/1）group were significantly increased by 38.18% and 58.22%（P 0.05）.Comprehensive antioxidant ability,digestive ability and immunity adding 13% of duckweed feed was the most suitable for the growth of Tajsho-sanke.