不同提取方法的紫地榆提取物对常驻口腔细菌的研究

Effect of Geranium Strictipes extracts extracted by different methods on acid production of three strains of oral bacteria

目的研究不同方法提取紫地榆正丁醇部分和乙酸乙酯部分对变形链球菌、黏性放线菌和血链球菌生长及产酸的影响。方法首先分别测定不同方法提取的紫地榆正丁醇部分和乙酸乙酯部分对变形链球菌、黏性放线菌及血链球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),再以低于MIC的4个浓度配制含药的胰酶解酪蛋白-植物蛋白胨-酵母提取物液体培养基,测定紫地榆正丁醇部分和乙酸乙酯部分对3种常驻口腔细菌产酸的影响。结果热提的紫地榆正丁醇部分对变形链球菌、黏性放线菌及血链球菌的MIC分别为3.0、3.0和6.0 g/L,MBC分别为6.0、6.0和12.0 g/L。热提的紫地榆乙酸乙酯部分对变形链球菌、黏性放线菌及血链球菌的MIC均为6.0 g/L,MBC均为12.0 g/L。冷提的紫地榆正丁醇部分对变形链球菌、黏性放线菌及血链球菌的MIC均为6.0 g/L,MBC均为12.0 g/L。冷提的紫地榆乙酸乙酯部分对变形链球菌、黏性放线菌及血链球菌的MIC均为6.0 g/L,MBC均为12.0 g/L。一定浓度的紫地榆正丁醇部分和乙酸乙酯部分对3种常驻口腔细菌的产酸有一定的抑制作用,但提取方法的不同对其抑制产酸的能力差异无统计学意义。结论紫地榆正丁醇部分和乙酸乙酯部分能有效抑制口腔致龋菌的生长和产酸,可能是一种潜在的天然防龋药物。

Objective To inquire into the effect of N-butyl alcohol and ethyl acetate extracts of Geranium Strictipes extracted by different methods on growth and acid production of Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus. Methods Firstly, minimum bacteriostatic concentration（MIC） and minimum bactericidal concentration（MBC） of N-butyl alcohol and ethyl acetate extracts of Geranium Strictipes extracted by different methods on Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus were respectively determined and then the effect of these Geranium Strictipes extracts on the acid production was determined. Results The MIC of N-butyl alcohol extract of Geranium Strictipes extracted reflux on Streptococcus mutans, Streptococcus sanguis and Actinomyces viscosus were 3.0, 3.0 and 6.0 g/L respectively, while the MBC were 6.0, 6.0 and 12.0 g/L respectively. The MIC of ethyl acetate extract of Geranium Strictipes extracted reflux on the three bacteria strains were all 6.0 g/L, and the MBC were all 12.0 g/L. The MIC of N-butyl alcohol extract of Geranium Strictipes extracted maceration on the three bacteria strains were all 6.0 g/L, the MBC were all 12.0 g/L. The MIC of ethyl acetate extract of Geranium Strictipes extracted maceration on the three bacteria strains were all 6.0 g/L, the MBC were all 12.0 g/L. A certain concentration of N-butyl alcohol and ethyl acetate extracts of Geranium Strictipes could inhibit acid production of the three bacteria strains,but there was no significant difference in the inhibition of acid production by different extraction methods.Conclusions N-butyl alcohol and ethyl acetate extracts of Geranium Strictipes can effectively inhibit the growth and acid production of oral bacteria, may be effective anti-caries agents.