超临界CO_2萃取银杏叶中总黄酮醇苷的夹带剂工艺条件

Process condition of entrainer in extracting total favonol glycosides from Ginkgo leaves by supercritical CO_2

目的探讨夹带剂在超临界二氧化碳CO_2中萃取银杏叶总黄酮醇苷的工艺条件。方法以夹带剂加入方式、夹带剂种类、夹带剂加入量及夹带剂流速为考察因素,总黄酮醇苷的提取率作为考察指标,用L9(34)正交表优化工艺条件,高效液相色谱法测定总黄酮醇苷含量。结果夹带方式选择预浸+动态萃取模式,以95%乙醇作为夹带剂,加入量300 ml,加入流速10 ml/min。在上述条件进行超临界萃取时,总黄酮苷醇提取率可达5.03%:萃取压力20 MPa,萃取温度60℃,出口温度70℃,用95%乙醇液态收集萃取物,动态萃取时间1 h。结论该实验优选的工艺明显提高超临界CO_2萃取银杏叶中总黄酮醇苷的提取效率。

Objective To research the process condition of entrainer in extracting total favonol glycosides from Ginkgo leaves by supercritical CO2. Methods The optimum extraction technology condition was investigated by orthogonal experiment [L9（34）] with extraction rate of total favonol glycosides as the evaluation index, and with the addition way, kind, amount and flow rate of entrainer as investigation factors. The content of total favonol glycosides was determined by high performance liquid chromatography（HPLC）. Results The adding way of entrainer is the presoak treatment plus dynamic extraction type, the entrainer was 95% alcohol,with 300 ml infusing volume and 10 ml/min flowing rate. The best factors of supercritical fluid extraction were as follows： extraction pressure 20 MPa, extraction temperature 60℃, outlet temperature 70℃, collecting extracts with 95% alcohol, dynamic extraction time 1 h. Under the processing conditions, the extraction rate of total favonol glycosides was 5.03%. Conclusions Supercritical CO2 extraction with the optimized process significantly improves the extraction of total flavonol glycosides from Ginkgo leaves.