AMPK、NF-κB、COX-2及PGE2在非小细胞肺癌中的表达

Expressions of AMPK, NF-κB, COX-2 and PGE2 in non-small cell lung cancer

目的探讨腺苷酸活化蛋白激酶(AMPK)、核因子κB(NF-κB)、环氧合酶2(COX-2)及前列腺素E2(PGE2)在非小细胞肺癌(NSCLC)中的表达及其关系。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法检测NSCLC和正常肺组织标本中可以直接反映AMPK激活状态的乙酰辅酶A羧化酶磷酸化后的产物(P-ACC)、NF-κB、COX-2的表达,实时荧光定量聚合酶链反应(qRT-PCR)和Western blot检测NSCLC标本及血液、正常肺组织标本及血液中AMPK、COX-2及PGE2受体的表达。结果免疫组织化学法检测发现,P-ACC在NSCLC中低表达,NF-κB和COX-2在NSCLC中高表达,其阳性率分别为33.9%、75.8%和66.1%,且与正常肺组织中的表达差异有统计学意义;qRT-PCR和Western blot检测发现,在NSCLC标本及血液中,AMPK mRNA表达降低,COX-2和PGE2受体mRNA表达升高。结论在NSCLC中,AMPK低表达,NF-κB、COX-2及PGE2高表达,其相互作用可能在NSCLC的增殖、凋亡耐受、侵袭及转移过程中占据重要位置,其表达情况为临床治疗NSCLC提供新的思路。

Objective To discuss the expressions and relations of AMP-activated protein kinase（AMPK）,nuclear factor-κB（NF-κB）, cyclooxygenase 2（COX-2） and prostaglandin E2（PGE2） in non-small cell lung cancer（NSCLC）. Methods The expressions of phosphorylation product of acetyl coenzyme A carboxylase（PACC, which could directly reflect active AMPK）, NF-κB and COX-2 in NSCLC and normal lung tissue specimens were detected by immunohistochemical SP method. The expressions of AMPK, COX-2 and EP2 in NSCLC, normal lung tissues and blood specimens were detected by qRT-PCR and Western blot. The SPSS22.0 software package was used for statistical analysis. Results The positive expression rate of P-ACC in the NSCLC specimens was 33.9% which was significantly lower than that in the normal lung tissue; while the positive expression rates of NF-ΚB and COX-2 in the NSCLC specimens were 75.8% and 66.1% respectively,which were significantly higher than those in the normal lung tissue. The expression of AMPK mRNA was reduced, while the expressions of COX-2 mRNA and PGE2 mRNA increased in the NSCLC specimens and blood, there were significant differences. Conclusions Low-expression of AMPK and high-expressions of NF-κB,COX-2 and EP2 in NSCLC may play important roles in NSCLC proliferation, apoptosis tolerance, invasion and metastasis. These different expressions provide a new idea for clinical treatment of NSCLC.