Th17细胞表面CCR4、CCR6、CXCR3表达量检测对慢性乙型肝炎患者病情评估的价值

Value of CCR4, CCR6 and CXCR3 expressions on peripheral blood Th17 cell surface in assessment of disease condition in chronic hepatitis B patients

目的分析外周血中辅助性T细胞17(Th17)表面CC趋化因子受体4(CCR4)、CC趋化因子受体6(CCR6)、CXC亚族趋化因子受体3(CXCR3)表达量检测对慢性乙型肝炎患者病情评估的价值。方法选取2013年8月-2015年12月在该院接受治疗的乙型肝炎患者118例作为观察组,同期在该院接受健康体检的肝功能正常人群142例作为对照组,对比两组外周血Th17细胞表面CCR4、CCR6、CXCR3表达水平,血清纤维化指标及肝功能指标,并进一步分析CCR4、CCR6、CXCR3表达量与慢性乙肝患者病情严重程度的相关性。结果慢性乙肝患者的外周血Th17细胞表面CCR4、CCR6、CXCR3表达水平,透明质酸(HA)、层黏连蛋白(LN)、Ⅳ型胶原(Ⅳc)、Ⅲ型前胶原(PCⅢ)值,丙氨酸转移酶(ALT)、白蛋白(Alb)、总胆红素(TBIL)值均高于对照组,球蛋白(Glo)值低于对照组,随着慢性乙肝病情加重,外周血Th17细胞表面CCR4、CCR6、CXCR3表达水平,HA、LN、Ⅳc、PCⅢ值,ALT、Alb、TBIL值进一步上升,Glo值下降。CCR4、CCR6、CXCR3表达量与慢性乙肝患者的外周血HA、LN、Ⅳc、PCⅢ、ALT、Alb、TBIL呈正相关,与Glo呈负相关(P<0.05)。结论慢性乙肝患者存在外周血Th17细胞表面CCR4、CCR6、CXCR3异常表达,其与患者病情严重程度直接相关,可以作为乙肝治疗的新靶点。

Objective To analyze the detective value of peripheral blood Th17 cell surface CCR4, CCR6 and CXCR3 expressions in assessment of disease condition in chronic hepatitis B patients. Methods Totally118 cases of hepatitis B patients in our hospital between August 2013 and December 2015 were chosen as observation group, 142 people having health examination in our hospital at same period as control group.CCR4, CCR6 and CXCR3 expression levels on peripheral blood Th17 cell surface, serum fibrosis markers and liver function indexes were compared between the two groups. The relationships of CCR4, CCR6 and CXCR3 expressions with disease severity were further analyzed. Results Th17 cell surface CCR4, CCR6 and CXCR3 expression levels in peripheral blood, serum HA, LN, collagen Ⅳ, PC Ⅲ, ALT, Alb and TBIL values in the observation group were higher than those in the control group, while Glo value was lower than that in the control group（P〈0.05）; these changes became more obvious along with exacerbation of chronic hepatitis B.CCR4, CCR6 and CXCR3 expressions in peripheral blood of the patients with chronic hepatitis B were positively correlated with HA, LN, collagen Ⅳ, PCⅢ, ALT, Alb and TBIL values, but negatively correlated with Glo value（P〈0.05）. Conclusions In chronic hepatitis B patients, Th17 cell surface CCR4, CCR6 and CXCR3 are abnormally expressed, and have direct correlations with severity of patients＇ condition. They can be used as new targets for the treatment of hepatitis B.