2014年肇庆市登革Ⅰ型病毒流行株E基因序列分析

Analysis on gene E sequence of Dengue typeⅠ virus strains in Zhaoqing during 2014

目的了解肇庆市2014年登革热病Ⅰ型病毒流行毒株E基因序列。方法收集肇庆市2014年登革热患者病例资料及急性期血清,用C6/36细胞培养分离登革热病毒,阳性分离株采用反转录-聚合酶链反应(RT-PCR)方法扩增E基因,绘制系统发生树,进行生物信息学分析。结果 36份标本中20份病毒分离培养阳性,获得肇庆市2014年Ⅰ型登革热病毒流行20株毒株的E基因序列,其同源性与中山市的2株流行毒株接近,但与广州市Ⅰ型登革病毒流行株相差较远。结论肇庆市登革热疫情同中山市登革热流行程度相关联且流行特点为输入性流行。

Objective To understand the gene E sequences of prevalent strains of Dengue fever type Ⅰ virus in Zhaoqing City during 2014.Methods The medical record data and acute stage serum of the patients with Dengue fever in Zhaoqing City during2014 were collected.Dengue virus was cultured and isolated by using the C6/36 cell culture.Gene E of positive strains was amplified with RT-PCR.The phylogenetic tree was drawn and the bioinformatics analysis was conducted.Results Of 36 samples,20samples were positive in viral isolation and culture.The gene E sequences of 20 strains of typeⅠ Dengue virus prevalence in Zhaoqing during 2014 were obtained;the homology of these sequences was close to that of the 2prevalent strains found in Zhongshan City,but was distant from that found in Guangzhou City.Conclusion The epidemic situation of Dengue fever in Zhaoqing City is closely related to the prevalence situation of Zhongshan and is characterized by imported prevalence.