量子点荧光猝灭法测定太湖河蚌中微囊藻毒素-RR

Determination of Microcystin-RR in River Clam of Taihu Lake Based on Fluorescence Quenching of Quantum Dots

建立了一种量子点(QDs)荧光猝灭法测定河蚌中微囊藻毒素-RR(MC-RR)的方法。将QDs与MC-RR单克隆抗体共价结合,形成QDs-MC-RR单克隆抗体生物共轭体。加入MC-RR,该生物共轭体发生荧光猝灭现象。在pH 7.17、反应温度为37℃、QDs浓度为2.0 mg/m L条件下,生物共轭体荧光猝灭程度和MC-RR浓度呈较好的线性关系。结果显示,该方法的检出限可达4.5×10^(-10)mol/L,相对标准偏差为8.9%,加标回收率为76.4%~87.3%。该方法操作简便、快速,可用于河蚌中微囊藻毒素-RR的检测。

To establish a method for the determination of microcystin-RR（MC-RR） in river clam by quantum dots（QDs） fluorescence quenching. Monoclonal anti-MC-RR antibody was conjugated to the modified QDs to form the bioconjugates. The fluorescence intensity of the bioconjugates between QDs and monoclonal anti-MC-RR antibody was quenched in the presence of MC-RR.Under temperature of 37 ℃,to an aqueous QDs solution（pH 7. 17） with the concentration of 2. 0 mg/m L,a linear relationship between the fluorescence quenching percentage of the bioconjugates and the concentration of MC-RR was observed. The detection limit of method was 4. 5 × 10-（-10） mol/L with relative standard deviation of 8. 9%. The average recoveries were 76. 4% - 87. 3%.The method is fast,simple and it could be used for the determination of microcystin-RR in river clam.