摘要
甜菊醇是新型甜味剂甜菊糖在人体内的代谢产物,在酸性环境中可重排成异甜菊醇,它们都是重要的药物合成中间体。研究甜菊醇和异甜菊醇与DNA的作用方式有助于理解其药理或细胞毒性。以小牛胸腺DNA(CT-DNA)为模板,通过考察甜菊醇和异甜菊醇对CT-DNA熔点和粘度的影响,以及甜菊醇和异甜菊醇与CT-DNA体系紫外吸收的变迁,初步推断甜菊醇和异甜菊醇都是以沟槽方式与CT-DNA结合;异甜菊醇与CT-DNA的作用比甜菊醇与CT-DNA作用强。
This work reported the interaction between steviol or isosteviol and CT-DNA by observing the changes on UV,viscosity and melting point of the CT-DNA before and after the interaction. Based on the results,it could be inferred that the binding of steviol and isosteviol with CT-DNA is a groove binding,and the interaction between isosteviol and CT-DNA is stronger than that of steviol and CT-DNA.
作者
李建
隋晓辰
王小侠
张珏
SUI Xiao-chen WANG Xiao-xia ZHANG Jue(Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China State Key Laboratory of Food Science and Technology, College of Chemical ＆ Material Engineering, Jiangnan University, Wuxi 214122, China)
出处
《化学试剂》
CAS
北大核心
2017年第2期187-190,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(31171752
31371837)
