HPLC法同时测定益心血脂康胶囊中3种成分

Simultaneous determination of three constituents in Yixin Xuezhikang Capsules by HPLC

目的建立HPLC法同时测定益心血脂康胶囊(黄芪、三七、川芎)中3种成分的含有量。方法该药物甲醇提取液的分析采用Agilent C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.2%甲酸为流动相,梯度洗脱;体积流量1 m L/min;检测波长260 nm(毛蕊异黄酮葡萄糖苷和芒柄花素)和321 nm(阿魏酸);柱温35℃。结果毛蕊异黄酮葡萄糖苷、芒柄花素、阿魏酸分别在0.084 4~0.844 0μg(r=0.999 8)、0.052 0~0.520 0μg(r=0.999 1)、0.043 4~0.434 0μg(r=0.999 2)范围内线性关系良好,平均加样回收率(RSD,n=6)分别为99.96%(2.65%)、96.30%(2.31%)、98.50%(2.46%)。结论该方法简便准确,重复性好,可用于益心血脂康胶囊的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of three constituents in Yixin Xuezhikang Capsules（ Astragali Radix,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma）.METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent C18column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 2% formic acid flowing at1 m L / min in a gradient elution manner,and the detection wavelengths were set at 260 nm for calycosin-7-Ο-β-Dglucoside and formononetin,and 321 nm for ferulic acid. RESULTS Calycosin-7-Ο-β-D-glucoside,formononetin and ferulic acid showed good linear relationships within the ranges of 0. 084 4- 1. 688 0 μg（ r = 0. 999 8）,0. 052 0- 0. 520 0 μg（ r = 0. 999 1） and 0. 043 4- 0. 434 0 μg（ r = 0. 999 2）,whose average recoveries（ RSDs,n = 6） were 99. 96%（ 2. 65%）,96. 30%（ 2. 31%） and 98. 50%（ 2. 46%）,respectively. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Yixin Xuezhikang Capsules.