血清IL-2、TNF-α及IL-13在类风湿关节炎诊断与治疗中的临床意义

Clinical significance of serum IL-2,TNF-α and IL-13 of patients with rheumatoid arthritis in the diagnosis and treatment of the disease

为探讨血清IL-2、TNF-α及IL-13在类风湿关节炎(rheumatoid arthritis,RA)诊断与治疗中的临床意义,收集了30例RA患者外周血,其中经过常规治疗的患者27例,常规治疗合并生物制剂治疗的患者3例,以30例健康体检者为正常对照,采用酶联免疫吸附试验双抗体夹心法检测RA患者治疗前后血清中IL-2、TNF-α及IL-13的表达水平,并分析IL-2、TNF-α及IL-13与临床指标RF、CRP、ESR的相关性。结果发现RA患者治疗前血清IL-2、TNF-α及IL-13表达水平明显高于健康对照组(P<0.05),常规治疗后IL-2表达水平降低(P<0.05),常规治疗合并生物制剂治疗后IL-2、IL-13表达水平明显降低(P<0.05);RA患者发病期血清IL-2、TNF-α及IL-13与RF、CRP、ESR呈正相关(P<0.05)。该研究提示RA患者血清中IL-2、TNF-α及IL-13的表达在RA的诊断与治疗中有一定的临床意义。

To investigate the clinical significance of IL-2, TNF-α and IL-13 in the serum of rheumatoid arthritis （RA） patients, the concentrations of these cytokines in the patients＇serum were measured from prior and post conventional treatment and or conventional plus biological agent treatment,and to analyze the correlations among IL-2,TNF-α,IL-13 and RA clinical index（ RF,CRP,ESR）. Thirty cases of RA patients from Ningxia medical university general hospital were enrolled,among them , 27 patients received conventional treatment and the remaining 3 cases received biological treatment in addition to conventional treatment. ELISA method was used to examine the concentrations of IL-2, TNF-α and IL-13. And then to analyze their correlation with RF, CRP and ESR. It was found that the expressions of IL-2, TNF-α and IL-13 in the serum from RA prior treatment group were significantly higher than those of the healthy control group. Post conventional treatment,IL-2 level was decreased. Post combined treatment,IL-2 and IL-13 levels were significantly decreased. During RA period,IL-2, TNF-α and IL-13 had obvious positive correlation with RF, CRP and ESR. Our results suggest that IL-2,TNF-α and IL-13 are not only important diagnosis indexes of RA,but also have clinic values in evaluating efficacy of biological agent treatment.