碳源自供给潜流人工湿地中反硝化基因与碳氮比的关系

Relationships Between Denitrifying Genes and C/N Ratio in Self-supplying Carbon Source Constructed Wetlands

本研究构建了4套碳源自供给水平潜流人工湿地装置,以香蒲生物质发酵液作为外加碳源,考察了生物质发酵液投加量(进水碳氮比CODAdd/NO_3-N=1,2,3,4)与植物(Typha latifolia)对反硝化功能基因nirS、nirK和nosZ的影响。结果表明,三种基因在湿地系统中数量级分别为107~108、105和10~6~10~7拷贝数/g砾石。生物质发酵液能促进含nirS、nirK和nosZ的微生物生长;植物能促进含nirS、nirK微生物生长。

In this study,four HSSF CWs were set up to investigated the effects of plant fermentation broth （ CODAdd/NO3-N = 1,2,3,4 ） and plants （Typha latifolia） on the denitrification genes. The results showed that the magnitude of copy numbers of the three genes in the wetland systems were 10^7 - 10^8 ,10^5 and 10^6 - 10^7 copies g^-1 The addition of fermentation broth could promote the growth of bacteria containing nirS,nirK and nosZ. Typha latifolia enhanced the growth of bacteria containing nirS and nirK.