干扰人整合素蛋白β5基因表达对瘢痕疙瘩成纤维细胞增殖能力的影响

The effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts

目的观察干扰人整合素蛋白β5基因对瘢痕疙瘩成纤维细胞增殖能力的影响，探讨其在瘢痕疙瘩发病中的作用。方法构建干扰整合素蛋白β5慢病毒载体并感染瘢痕疙瘩成纤维细胞，通过PCR及WesternBlot方法观察感染后细胞整合素蛋白β5基因和蛋白的表达情况；MTT法检测不同时间点细胞的增殖情况。结果慢病毒感染瘢痕疙瘩成纤维细胞后，空白对照组、空载病毒组及慢病毒干扰组中整合素蛋白β5mRNA及蛋白的表达量分别为1．00±0．00、1．08±0．05、0．34±0．01和0．91±0．03、0．93±0．02、0．28±0．07，与空载病毒组及空白对照组比较，慢病毒干扰组中整合素蛋白85mRNA及蛋白的表达量均明显降低，差异有统计学意义（P〈0．01）；慢病毒感染瘢痕疙瘩成纤维细胞后，各组间细胞的增殖情况在接种24h后无显著差异，48h后慢病毒干扰组的增殖较其他2组明显变慢（P〈0．01）。结论整合素蛋白β5基因可促进瘢痕疙瘩成纤维细胞的增殖。

Objective To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of [35-integrin（ ITGB5 ） in keloid. Methods Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT. Results The expression quantity of ITGB5 mRNA and protein in KFb group, LV-NC group and LV-KFb group are 1.00 ± 0.00, 1.08 ±0.05, 0.34 ±0.01 and 0.91 ±0.03, 0.93 ±0.02,0.28 ±0.07. Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV- KFb group decreased significantly （P 〈 0. 01 ）. Compared with LV-NC group and KFb group, the proliferation rate decreased significantly in LV-KFb group at 48 h（P 〈0. 01 ）. Conclusions These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids. [