原头蚴体外刺激巨噬细胞高表达PPARα/γ并调节其极化

Echinococcus granulosus stimulates high PPARα/γ expression and drives polarization of macrophages in vitro

目的:探讨原头蚴与RAW264.7共培养后PPARα/γ的表达水平以及对巨噬细胞极化的作用。方法:原头蚴与RAW264.7共培养12、24、36、48、72 h取细胞。用qRT-PCR的方法检测RAW264.7细胞PPARα/γ的表达水平,用qRTPCR的方法检测巨噬细胞M1型相关因子TNF-α、MCP-1、IL-1β和M2型相关因子Arg-1、TGF-β、Fizz-1的水平,ELISA的方法检测Arg-1和MR的表达量。结果:RAW264.7细胞PPARα/γ的表达水平和M2型相关因子Arg-1、TGF-β、Fizz-1和MR均逐渐升高,72 h表达最高(P<0.05);巨噬细胞M1型相关因子TNF-α、MCP-1、IL-1β先升高后降低(P<0.05),Arg-1和MR的蛋白水平也逐渐增高。结论:原头蚴与RAW264.7共培养后PPARα/γ的表达逐渐升高,可能促进巨噬细胞由M1型向M2型极化,从而在虫体免疫逃逸中发挥一定作用。

Objective： To investigate the expression levels of PPARα/γ in RAW264. 7 cells in the early stages of co-cultivation with Echinococcus granulosus in vitro. Methods： RAW264. 7 cells were co-cultured with E. granulosus and collected at 12,24,36,48,72 h. The mRNA levels of PPAR-γ,PPAR-α,M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β,and M2 macrophages-associated cytokines including Arg-1,TGF-β and Fizz-1 were detected by qRT-PCR. The protein levels of Arg-1 and MR were analyzed by ELISA. Results： The expression levels of PPAR-γ,PPAR-α and the M2 macrophages-associated cytokines including Arg-1,TGF-β,Fizz-1 and MR were significantly increased,especially at 72 h（ P 0. 05）. M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β were decreased at 72 h although increased at first. Conclusion： During the early stages of co-cultivation with Echinococcus granulosus in vitro,the levels of PPAR-γ / α are up-regulated in RAW264. 7 cells,which may drive macrophage polarization and play a role in the immune escape.