扁蒴藤素对支气管哮喘患儿Th17/Treg失衡的影响及意义

Influence and significance of pristimerin therapy in patients with allergic asthma on Th17 and Treg

目的:探讨扁蒴藤素对支气管哮喘患儿Th17/Treg体外培养细胞的影响及意义,从而探讨免疫因素在支气管哮喘治疗中的机理。方法:提取支气管哮喘患儿外周血单个核细胞,并在体外给予扁蒴藤素干预,流式细胞术检测Th17及Treg比例,ELISA方法检测细胞培养上清液中IL-17及TGF-β含量变化,实时定量PCR检测RORγT及Foxp3含量。结果:扁蒴藤素干预组与对照组相比,CD4^+IL-17^+T细胞(即Th17细胞)表达量显著降低,CD4^+CD25^+Foxp3^+T细胞(即Treg细胞)表达量显著升高,各组均存在统计学意义(P<0.05);与对照组相比,IL-17含量显著降低,TGF-β含量显著升高,各组均存在统计学意义(P<0.05);与对照组相比,RORγT含量显著降低,Foxp3含量显著升高,各组均存在统计学意义(P<0.05)。结论:扁蒴藤素干预可较大程度缓解体外培养Th17/Treg细胞失衡,提示该药物可能通过影响免疫平衡来调节哮喘的发生发展,为扁蒴藤素在临床治疗哮喘的应用提供了理论支持。

Objective： To investigate the effects of pristimerin therapy on the functions of Th17 and Treg cells in patients with allergic asthma,and expore the function of immune factors in therapy on allergic asthma. Methods： Extracted mononuclear cells in peripheral blood of patients with allergic asthma,and given pristimerin intervention in vitro. Then used fluorescence-activated cell sorting analysis to detect the contect of Th17 and Treg,enzyme-linked immunosorbent assay to detect cytokine production of IL-17 and TGF-β,and Real-time PCR to detect the contect of RORγT and Foxp3. Results： Compared with the control group,experimental group had reduced expression of CD4~＋IL-17~＋T cells（ Th17 cells）,and increased expression of CD4~＋CD25~＋Foxp3~＋T cells（ Treg cells）,which all had the statistical significance（ P〈0. 05）; displayed decreased level of IL-17,and increased level of TGF-β,which all had the statistical significance（ P〈0. 05）; had reduced expression of RORγT,and increased expression of Foxp3,which all had the statistical significance（ P〈0. 05）. Conclusion： Pristimerin therapy can remit the unbalance of Th17 / Treg in vitro,hinting this drug may regulate allergic asthma by influencing immunologic balance,which offers the theoretical support for pristimerin in clinical application.