南五味子多糖急性毒性及对小鼠肝损伤保护作用的研究

Acute Toxicity and Protective Effects of Polysaccharide from Schisandrae Sphenantherae Fructus in Mice with Liver Injury

目的:研究南五味子多糖的急性毒性及对四氯化碳(CCl_4)致小鼠肝损伤的保护作用。方法:采用经典的急性毒性实验方法,测定南五味子多糖的最大给药量(MLD),并观察其急性毒性反应;采用0.5%CCl_4致小鼠急性肝损伤模型,将60只小鼠随机分为空白对照组、CCl_4模型对照组、联苯双酯组及南五味子多糖高(1 388 mg·kg^(-1))、中(694 mg·kg^(-1))、低(347 mg·kg^(-1))剂量组,每组10只,连续给药6 d,检测血清中AST和ALT水平,观察肝脏的损伤程度。结果:急性毒性实验中对小鼠的一般行为、体质量变化及各脏器检查均未见异常,南五味子多糖毒性症状不显著;与模型对照组比较,南五味子多糖3个剂量组的ALT水平显著降低(P<0.05或P<0.01),南五味子多糖高、中剂量组的AST水平显著降低(P<0.01),一定程度上改善了肝脏组织损伤。结论:南五味子多糖无显著毒性,对CCl_4致小鼠急性肝损伤具有一定的保护作用。

Objective： To evaluate the acute toxicity and protective effects of polysaccharide from Schisandrae sphenantherae Fruc- tus in the mice with acute liver injury induced by CCl4. Methods： The classical acute toxicity tests were used to determine the maxi- mum drug loading （MLD） and observe the acute toxicity. The acute liver injury model was induced by 0.5% CCl4. Sixty mice were randomly divided into the blank control group, CCI4 model group, bifendatatum model group and polysaccharide group respectively at high （1 388 mg. kg-1）, medium （694 mg. kg-1） and low （347 mg kg 1） close groups with 10 ones in each. After 6-day drug treatment, the levels of aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were determined by kits. Histopatho- logical examination was also carried out. Results： General behavior, body weight and organ examination in the acute toxicity tests showed no obvious changes. Compared with those in the model group, the levels of AST in the three polysaccharide groups significantly decreased （P 〈0.05 or P 〈0.01 ） , the levels of ALT in polysaccharide group at high and medium doses significantly decreased（P 〈 0.01 ）, and the liver tissue damage was improved to a certain extent as well. Conclusion ： Polysaecharide from Schisandrae sphenan- therae Fructus has protective effects on acute liver injury induced by CC14 in mice with low acute toxicity.